肉鸡养殖场以心包积液为特征病例的诊断

Diagnosis of an Infectious Disease Characterized with Hydropericardium in Broiler Farm

自2014年以来,出现了一种以发生于28~35日龄、发病急、病死率高、剖检可见心包积液、肝脏坏死、肾脏肿大、出血为特征的鸡急性传染病的病例。本研究通过其中一例典型病例的病原分离鉴定研究,发现禽腺病毒检测为阳性。进一步通过禽腺病毒的动物回归试验、病理组织切片分析等,结果发现,该病毒可引起鸡出现相似的临床发病特征和病理变化特征。通过对病毒的hexon基因序列及推导的氨基酸序列进行分析,其与血清4型的同源性最高。因此,确定该病的病原为禽腺病毒血清4型,也确认鸡心包积水综合征在我国肉鸡场的发生和流行。

Since 2014, we have noticed that a new chicken disease emerged in the broiler farms,which is characterized by an acute outbreak at 28-35 days of age, high mortality With pericardial effusion,liver necrosis, renal swelling and bleeding. In the present study, one of the typical case of the diseases was selected for the identification of the pathogen. Fowl adenovirus was detected by PCR. The isolated virus caused similar clinical signs and pathological changes in chickens during the animal regression test. According to gene sequence and the deduced amino acid sequence of hexon, the isolated virus showed high homology to serotype 4. Based on the study, we conclude that the pathogen of the hydrocephalus syndrome is fowl adenovirus serotype 4.