淫羊藿苷对前列腺癌细胞株活力、迁移与侵袭的作用

Effects of icariin on viability,migration and invasion of prostate cancer cells

目的:探讨淫羊藿苷对前列腺癌细胞株Du145与PC3的存活、迁移与侵袭能力的影响,并初步研究其机制。方法:CCK-8法检测不同浓度淫羊藿苷(0、5、10、20、40和80μmol/L)对Du145细胞与PC3细胞活力的影响;Transwell小室实验检测细胞的迁移和侵袭能力;Western blot检测Notch-1、MMP-2、MMP-9和Hes-1蛋白水平。结果:MTT法检测结果显示淫羊藿苷呈浓度依赖性抑制Du145与PC3细胞的活力,当浓度到达40μmol/L时,抑制作用达到最大;经淫羊藿苷干预后,Du145和PC3细胞迁移和侵袭能力显著下降,Notch-1、MMP-2、MMP-9和Hes-1蛋白表达水平显著下降。结论:淫羊藿苷具有抑制前列腺癌细胞株Du145与PC3生长、迁移和侵袭的作用,其作用机制可能与淫羊藿苷抑制Notch-1、MMP-2、MMP-9和Hes-1蛋白的表达有关。

AIM： To investigate the effects of icariin on the viability, migration and invasion of the prostate cancer lines Dul45 and PC3. METHODS： Dul45 cells and PC3 cells were treated with icariin at different concentrations （0,5, 10, 20, 40 or 80 jjimol/L） , and the cell viability was measured by CCK-8 assay. The cell migration and invasion abilities were detected by Transwell assay. The protein expression of Notch-1, matrix metalloproteinase （ MMP） -2, MMP-9 and hairy/enhancer of split-1 （ Hes-1） was determined by Western blot. RESULTS ： The results of MTT assay revealed that icariin inhibited the viabilitiy of Dul45 cells and PC3 cells in a dose-dependent manner. The maximal effect was at dose of 40 jjimol/L. Icariin treatment significantly decreased the abilities of migration and invasion of Dul45 cells and PC- 3 cells. Moreover, the protein expression of Notch-1, MMP-2, MMP-9 and Hes-1 was dramatically reduced after icariin treatment. CONCLUSION ： Icariin inhibits prostate cancer cell viability, migration and invasion by decreasing the protein expression of Notch-1, MMP-2, MMP-9 and Hes-1.