Netrin-1促进脑缺血康复的作用机制研究

Study of NT-1 in Promoting the Recovery Mechanism in MCAO Model

目的探讨NT-1蛋白能否促进脑缺血的康复及其可能机制。方法 30只ICR小鼠,被随机分成NT-1组以及BSA组各15只,分别给予NT-1及BSA脑内立体定位注射。采用线栓法制作大脑中动脉缺血模型,采用NRSS评分以及平衡木实验观察动物缺血后行为学康复情况,采用免疫组织化学染色的方法评价血管新生以及细胞凋亡情况。结果行为学结果显示与BSA组比较,NT-1能够降低NRSS行为学得分,NT-1能够降低动物跑过平衡木所需要的时间。免疫组织化学结果显示,与BSA组相比较NT-1增加了血管的数量,减少了凋亡细胞的数量。结论 NT-1蛋白脑内注射促进中风的康复,其可能机制是通过促进血管新生以及抑制凋亡。NT-1蛋白是一潜在的治疗脑缺血的药物。

Objective To explore the study of NT - 1 promote recovery mechanisms after cerebral ischemia. Meth-ods 30 ICR mice were equally divided into NT - 1 and BSA groups and respectively given NT - 1 and BSA injection in the brain. The middle cerebral artery ischemia model was established with suture method. NRSS score and beam - walking test were used to exam the behavior performance after MCAO. Immnuohischemstry was used to evaluate the status of angiogenesis and apoptosis. Results Compared to the BSA group, NT - 1 not only reduced the time on the beam - walking but the score of NRSS behavioristices. NT - 1 also increased the number of vessels and reduced the number of apoptotic cells. Conclusion NT - 1 cerebral injection can promote the recovery by increasing angiogenesis and inhibiting apoptosis. NT - 1 is a promise target in the treatment of cerebral ischemia.