肠道病毒核酸与抗体联合检测在手足口病诊断中的应用价值研究

Application value of combined detection of virus nucleic acid and antibody in HFMD

目的探讨肠道病毒71型(EV71)核酸与抗体联合检测在手足口病诊断中的应用价值。方法于2015年1-11月,将108例疑似手足口病患儿随机分为核酸检测组、抗体检测组和联合检测组,每组各36例。疱疹破裂渗出液病毒核酸检测采用逆转录聚合酶链反应法,血清病毒抗体检测采用酶联免疫吸附法,分析各组及不同症状患儿检测阳性率。结果核酸检测组阳性率最高(80.55%),抗体检测组阳性率最低(47.22%)。不同症状患儿中,皮疹患儿核酸、抗体及联合检测阳性率最高。结论 EV71病毒核酸及抗体联合检测可避免核酸单独检测阳性结果和抗体单独检测假阴性结果,有助于提高手足口病的诊断准确率。

Objective To investigate the application value of combined detection of enterovirus 71 type(EV71)nucleic acid and antibody in diagnosis of hand-foot-mouth disease(HFMD).Methods From Jan.to Nov.2015,a total of 180 children with suspected HFMD were divided into nucleic acid detection group,antibody detection group and combined detection group,with 36 subjects for each group.Viral nucleic acid was detected by using reverse transcription polymerase chain reaction.Antibody was detected by using enzyme linked immunosorbent assay.All detected results were analyzed.Results The positive rate of nucleic acid detection group was the highest(80.55%),while that of antibody detection group was the lowest(47.22%).Among various symptoms,the positive rates of nucleic acid,antibody and combined detection in subjects with skin rash were the highest.Conclusion Combined detection of EV71 nucleic acid and antibody could avoid the false positive result of nucleic acid detection and false negative result of antibody detection,thus could be helpful for increasing diagnostic accuracy rate of HFMD.