咪唑离子液体为背景HPCE测定血清中头孢药物

Simultaneous Determination of Cephalosporins in Serum by High Performance Capillary Electrophoresis with Ionic Liquid as Background

建立了以[Bmim][BF4]离子液体为背景电解质,高效毛细管电泳同时测定血清中头孢噻肟(CTX)、头孢唑啉(CFZ)、头孢曲松(CRO)和头孢米诺(CMN)这4种头孢类药物的方法。将头孢拉定(RAD)作为内标,考察了背景电解质浓度、分离电压、溶液p H、进样时间对分离度的影响。结果发现,背景电解质浓度为30 mmol/L、分离电压为15 kV、进样时间为10 s、检测波长为250 nm时,CTX、CFZ、CRO和CMN在9 min内能得到有效地分离,在0.5~100.0 mg/L范围内有良好的线性关系(r>0.999),检出限分别为0.61、0.56、0.46和0.79 mg/L,定量限分别为2.05、1.90、1.56和2.65mg/L,精密度(RSD)<3.2%。该方法用于血清中头孢药物的分析,回收率为97.16%~101.40%。离子液体经CH2Cl2萃取回收,回收率达85%以上。

A capillary electrophoresis method has been developed for the simultaneous determination of four cephalosporins （ cefo- taxime（CTX） ,eefazolin（CFZ） ,ceftriaxone（CRO） and cefminox（CMN） ） with ionic liquid as background in serum. Using cefra- dine（RAD） as internal standard, the influence of the eleetrophoresis conditions（ concentration of background electrolyte, voltage, pH values and injection time） on the separation were investigated. The best result was obtained using 30 mmol/L [ Bmim] [ BF4 ] （ pH 10. 5） as background,at 15 kV,the injection time of 10 s, UV detection at 250 nm. The four cephalosporins were effectively separated in less than 9 minutes and good linear relationship （r 〉 0. 999 ） were obtained in the 0.5 - 100.0 mg/L range. The de- tection limits of four eephalosporins were 0.61,0. 56,0.46 and 0.79 mg/L,respeetively. The limits of quantification were 2.05, 1.90,1.56 and 2. 65 mg/L respectively and RSD 〈 3.2% . The method was applied to the determination of the cephalosporins in serum and recovery was in the range from 97.16% - 101.40%. Ionic liquids was extracted by CH2C12 and recovery was more than 85 %.