新疆石河子地区设施樱桃病毒病害调查及病原检测

Investigation and Pathogen Detection of Cherry Virus Disease in Greenhouses in Shihezi of Xinjiang

【目的】研究新疆石河子地区设施樱桃病毒病的发生情况,明确病毒种类及带毒率。【方法】对新疆石河子设施樱桃病毒病害进行调查,利用已报道在樱桃上发生的8种病毒特异性引物,对122个设施樱桃疑似病毒病样品进行RT-PCR检测及序列同源性分析。【结果】石河子地区设施樱桃病毒病田间症状主要表现有花叶、卷叶、褪绿黄化、畸形、皱缩等。在样品中扩增出与樱桃病毒A(Cherry virus A,CVA)、李属坏死环斑病毒(Prunus necrotic ring spot virus,PNRSV)、李矮缩病毒(Prune dwarf virus,PDV)和樱桃绿环斑驳病毒(Cherry green ring mottle virus,CGRMV)预期大小一致的目的片段;序列分析表明4种病毒扩增片段与Gen Bank中注册的相应病毒核苷酸和氨基酸序列均具有较高的同源性;其中分离物CVA-SHZ、PNRSV-SHZ、PDV-SHZ和CGRMV-SHZ与Gen Bank中已报道的分离物Ch TA12(KT310083.1)、DJ1-1(JF333586.1)、HSY4-1(HQ539657.1)、ZZ-Ch-13(KC965106.1)对核苷酸同源性分别为99.5%、99.5%、99.0%和99.4%。CVA、PNRSV、PDV和CGRMV的检出率分别为67.3%、61.1%、6.5%、4.9%,受两种及两种以上病毒复合侵染检出率56.5%。【结论】新疆石河子地区设施樱桃病毒病原以CVA和PNRSV为主,且两种病毒复合侵染较为普遍。这是在新疆设施樱桃上检测到CVA、PNRSV、PDV、CGRMV4种病毒。

[ Objective] In order to investigate the situation and identify the virus types of cherry virus diseases in greenhouse cultivation cherry in Shihezi of Xijiang. [ Method] Virus diseases were investigated in greenhouse cultivation cherry in Shihezi, eight pairs of specific primers were utilized to detect the 122 symptomatic samples by RT- PCR. [ Result] The results show that symptoms mainly included mosaic and green mottle, roll leaf, yellow, deformity, crinkle, etc. RT -PCR amplification showed that Cherry virus （CVA）, Prunus necrotic ring spot virus （PNRSV）, Prune dwarf virus（ PDV ） , Cherry green ring mottle virus （CGRMV） were found in the samples; The similarity analysis of the nucleotide sequences showed that isolates of CVA - SHZ, PNRSV -SHZ, PDV- SHZ and CGRMV- SHZ were reported in GenBank isolates ChTA12 （KT310083.1）, DJ1 - 1 （JF333586.1）, HSY4 - 1 （ HQ539657.1 ）, ZZ - Ch - 13 （ KC965106. 1 ）. And their nucleotide homologies were 99.5 %, 99.5 %, 99.0% and 99.4%, respectively. The average rates of in- fection with CVA, PNRSV, PDV and CGRMV were 67.3% , 66.1%, 6.5% and 4.9%, respectively. The average rates of infection with more than two viruses were 56.5% samples. [ Conclusion] CVA and PNRSV are the main viruses in greenhouse cherry in Shihezi of Xijiang, and the mixed infection of the two are common. This is the first report on the detection of CVA, PNRSV, PDV and CGRMV in cherry in Xinjiang.