miR-187在胃癌组织中的表达及意义

Expression of miR-187 in the tissues of gastric cancer and its clinical significance

目的本研究旨在探讨microRNA(miR)-187在胃癌组织中的表达情况,并分析miR-187表达的改变与胃癌分化程度和TNM分期之间的关系。方法 32例胃癌及相应癌旁正常胃黏膜组织标本提取RNA后,采用茎环逆转录实时荧光定量PCR(real time-PCR)方法,分别检测miR-187在胃癌及癌临近正常胃黏膜组织中的表达量,并进一步观察miR-187表达的变化同胃癌分化程度以及TNM分期之间存在的联系。结果胃癌及癌旁正常胃黏膜组织中均可以检测到miR-187,且与癌临近的正常胃黏膜组织相比,miR-187的表达量发生显著的改变,其在胃癌组织中表达出现显著性的下降(P<0.05)。胃癌组织中的miR-187表达量与胃癌的分化程度(P<0.05)及TNM分期(P<0.05)均显著相关。结论在胃癌组织及癌临近胃黏膜组织中,miR-187的表达呈明显减少的趋势,差异具有统计学意义;且胃癌组织中miR-187表达的减少与胃癌的分化程度以及TNM分期均呈显著的相关性。以上研究结果表明,miR-187表达的减少与胃癌的发生、发展密切相关。

Objective To investigate the expression of microRNA （miR）-187 and its association with clinicopathologic features in the gastric cancer and YNM staging. Methods The miR-187 expression in the 32 cases of gastric cancer and the matched non-tumor adjacent tissue specimens were examined by using stem-loop Real-Time PCR（RT-PCR）. The relationship between the expression of miR-187 and its clinicopathologic features of gastric cancer was analyzed by RT-PCR. Results miR-187 was detected in both the tumor tissues and the non-tumor adjacent tissues. The expression level of miR-187 in the tumor tissues was significantly lower than that in the adjacent tissues （P 〈 0.05 ）. Down-regulated rniR- 187 expression was associated with the cell differentiation（ P 〈 0.05 ） and lymph node metastasis TNM staging（ P 〈 0.05 ） in gastric cancer patients. Conclusions There is a significant difference in the expression level of miR-187 between the tumor tissues and the adjacent tissues（ P 〈 0.05 ）. The miR-187 expression was associated with the cell differentiation and lymph node metastasis TNM staging. The expression of miR-187 might play a key role in the carcinogenesis and progress of gastric cancer.