脑胶质瘤中胞质多聚腺苷酸化元件结合蛋白4的表达情况及其对胶质瘤细胞生长的影响

Expression of cytoplasmic polyadenylation-binding protein-4 in glioma and its effect on glioma cell growth

目的观察脑胶质瘤中胞质多聚腺苷酸化元件结合蛋白4(CPEB4)的表达情况,以及CPEB4对胶质瘤细胞U87生长的影响。方法测定脑胶质瘤和脑组织中CPEB4蛋白含量。测定细胞中CPEB4蛋白表达、细胞增殖、细胞凋亡和细胞周期及CPEB4、PIK3CA、IGF2、SMAD3、IDH1和AKT1 mRNA的表达。结果低级别脑胶质瘤组织和高级别脑胶质瘤组织中CPEB4蛋白阳性表达率高于正常脑组织(P<0.05),高级别脑胶质瘤组织中CPEB4蛋白阳性表达率高于低级别脑胶质瘤组织(P<0.05)。低级别脑胶质瘤组织和高级别脑胶质瘤组织中CPEB4蛋白水平高于正常脑组织(P<0.05),高级别脑胶质瘤组织中CPEB4蛋白水平高于低级别脑胶质瘤组织(P<0.05)。转染后24 h,CPEB4-shRNA组U87细胞中CPEB4蛋白水平低于阴性对照组和空白对照组(P<0.05)。培养第3天和第7天,CPEB4-shRNA组细胞的A490值低于阴性对照组和空白对照组(P<0.05)。CPEB4-shRNA组早期细胞凋亡率明显高于阴性对照组和空白对照组(P<0.05)。CPEB4-shRNA组G1期细胞明显高于阴性对照组和空白对照组(P<0.05),S期细胞明显低于阴性对照组和空白对照组(P<0.05)。CPEB4-shRNA组CPEB4 mRNA、PIK3CA mRNA、IGF2 mRNA和SMAD3 mRNA相对表达量均低于阴性对照组和空白对照组(P<0.05)。结论 CPEB4在脑胶质瘤中高表达,CPEB4参与脑胶质瘤细胞的生长,CPEB4对脑胶质瘤细胞的影响可能和CPEB4、PIK3CA、IGF2和SMAD3水平有一定关系。

Objective To observe the expression of cytoplasmic polyadenylation element binding protein 4 （CPEB4） in glioma and the effect of CPEB4 on the growth of glioma U87 cells. Methods The expression of CPEB4 protein in glioma tissues and normal brain tissues were detected. The expression of CPEB4 protein in cells, the cell proliferation, apoptosis, eel1 cycle and the expression of CPEIM,PIK3CA,IGF2, SMAD3,IDH1 and AKT1 mRNA were measured. Results The positive rate of CPEB4 protein in low-grade glioma gliomas tissues and high-grade gliomas tissues were higher than that in the normal brain tissues（P 〈0.05）. The positive rate of CPEB4 protein in the high grade glioma tissues was higher than that in the low grade glioma tissues （ P 〈 0.05 ）. The level of CPEB4 protein in the Low-grade glioma tissues and highgrade glioma tissues were significantly higher than those in the normal brain tissues. The level of CPEB4 protein in the high grade glioma was higher than that in the low grade glioma （ P 〈 0.05 ）. At 24 h after transfection, the level of CPEB4 protein in the CPEIM-shRNA group was lower than that in the negative control group and blank control group（ P 〈 0.05 ）. On the third and seventh day of culture, the A490 value of CPEB4-shRNA group were lower than those in the negative control group and the blank control group （ P 〈 0.05 ）. The early apoptotic rate in the CPEB4-shRNA group was significantly higher than that in the negative control group and the control group（ P 〈 0.05 ）. The G1 phase cells of CPEB4-shR- NA group was significantly higher than that the negative control group and the control group（ P 〈 0.05 ） ,the S phase cells was significantly lower than those in the negative control group and the blank control group （ P 〈 0.05 ）. The relative expression of CPEB4 mRNA, PIK3CA mRNA, IGF2 mRNA and SMAD3 mRNA in CPEB4-shRNA group were lower than those in negative control group and blank control group （ P 〈 0.05 ）. Conclusion The CPEB4 is highly expressed in gliomatissues, CPEB4 involves the growth of glioma cells. The effect of CPEB4 on glioma cells may be related to the levels of CPEB4,PIK3CA, IGF2 and SMAD3.