mro-miR-33在绿僵菌产孢中的作用

The effects of mro-miR-33 on the conidial production in Metarhizium robertsii

罗伯茨绿僵菌Metarhizium robertsii是一种重要的广谱性杀虫真菌,在害虫的生物防治中应用广泛。研究表明microRNAs在丝状真菌的生长发育中可能发挥重要调控作用。迄今有关miRNAs在绿僵菌产孢过程中的作用还未见研究报道。本文分析了实验室前期鉴定的一个绿僵菌新miRNA(mro-miR-33)在绿僵菌产孢过程中的作用。结果表明,绿僵菌在不同培养条件下mro-miR-33的表达量有显著差异,且在孢子发育过程表达量下降。在野生菌株中过表达mro-miR-33后,绿僵菌的产孢量显著下降,qRT-PCR分析表明孢子发育关键调控基因brl A表达显著下调,而敲除mro-miR-33后,绿僵菌的产孢量显著增加。这些变化表明mro-miR-33在绿僵菌的产孢过程中发挥着重要的作用。

Meterhizium robertsii is an important entomopathogenic fungus, widely used for control of agricultural pest insects and vectors of human diseases. Recent studies showed that microRNAs misht play important roles in fungal growth and development. However, the function of miRNAs in regulating sporulation in M. robertsii has not been reported. In this study, a novel miRNA, mro-miR-33, previously identified from our small RNA deep-sequencing libraries was down resulated during fungal sporulation. Over-expression of mro-miR-33 in wild type M. robertsii significantly reduced conidial production. The brlA gene resulating conidiophore development was significantly down-resulated in mro-miR-33 over-expressing strain. However, disruption of mro-miR-33 significantly increased conidial production compared to wild type. These results suggest that mro-miR-33 negatively regulates conidiation in Metarhizium robertsii.