一色齿毛菌CB1对活性染料的脱色研究

Cerrena unicolor CB1 capability in reactive dye decolorization

前期工作中采用组织分离法分离、纯化得到一白腐真菌CB1,本研究进一步观察菌株CB1形态特征和培养特性,克隆菌株CB1的ITS基因序列,对菌株CB1的ITS序列进行同源性比对和系统进化分析,完成菌株CB1综合的鉴定。同时对菌株CB1脱色两种活性染料进行了研究,探讨菌株CB1脱色活性染料的能力。结果表明,菌株CB1形态特征和培养特性与一色齿毛菌Cerrena unicolor特征基本吻合,菌株CB1的系统进化分析表明菌株CB1的ITS序列与一色齿毛菌的ITS序列有较近的进化关系,形态学和分子生物学综合鉴定菌株CB1为C.unicolor CB1。染料脱色结果表明,菌株CB1可以明显脱色活性黑和活性红染料,与脱色活性黑相比,菌株CB1可以更有效地脱色活性红染料,在脱色12d时,菌株CB1对浓度为10、50、100、250、500mg/L的活性黑脱色率分别为96%、93%、65%、55%、40%,菌株CB1对浓度为10、50、100、250、500mg/L的活性红在12d时的脱色率分别为98%、95%、91%、87%、83%。

A white rot fungus strain CB1 was isolated and purified. The strain shares highest similarity with Cerrena unicolor in morphology and culture characteristics as well as the ITS sequence. It shows remarkable capability of decolorizing reactive black and reactive red dye. The decolorization rate for reactive black dye was 96%, 93%, 65%, 55%, and 40% and for reactive red dye 98%, 95%, 91%, 87%, and 83% at the dye＇s concentration of 10, 50, 100, 250, and 500mg/L, respectively