癫痫清颗粒对大鼠海马区神经元影响

Protective Effect of Dianxianqing Granule on Pentylenetetrazole-induced Seizures in Rats

目的:观察癫痫清颗粒对戊四唑引起大鼠海马神经元凋亡的影响。方法:大鼠腹腔注射10 g·L^(-1)戊四唑(40 mg·kg^(-1)),每2 d 1次,连续15次,停药l周后,再次腹腔注射戊四唑,根据本次癫痫发作级别随机分为7组,每组6只,连续灌胃给药治疗4周,麻醉后取脑,通过尼氏染色观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型海马组织结构的影响,并通过免疫组化染色观察癫痫清颗粒对戊四唑诱发大鼠慢性癫痫模型海马组织神经元Apaf-1、caspase-9和caspase-3表达的影响。结果:与模型对照组相比癫痫清低、中、高剂量组及苯妥英钠组大鼠海马齿状回存活细胞显著升高,与模型对照组相比癫痫清低、中、高剂量组Apaf-1表达均显著降低,与模型对照组相比癫痫清中剂量组caspase-9表达显著降低,与模型对照组相比癫痫清低、中、高剂量组caspase-3表达均显著降低。结论:癫痫清颗粒对戊四唑诱发慢性癫痫模型大鼠海马区神经元具有呈剂量依赖性的保护作用。

Objective ： This study was to investigate the effect of Dianxianqing Granule （ DXQ ） on pentylenetetrazoleinduced hippocampal pyramidal cells apoptosis and cerebrum damage, then to explore its possible mechanisms. Methods：Rats were given 10 g·L^-1 pentylcnetetrazole intraperitoneal injection （ 40 mg·kg^-1）, 1 time/2 d, continuous 15 times. After withdrawal 1 weeks, given pentylenetetrazole intraperitoneal injection again, according to the epileptic seizure level, rats were divided into 7 groups, each group 6 cases,4 weeks later brain was taken after anesthesia. The number of surviving hippocampal pyramidal cells was assessed by Nissl staining. The expression of Apaf-1, caspase-9 and easpase-3 were analyzed byimmunohistoehemical （ IHC ） staining. Results ： Survival cells of dentate gyrus of hippocampus of DXQ low, middle, high dose group and dilantin group significantly increased compared with model group. Expression of Apaf-1 of DXQ low, middle, high dose group significantly decreased compared with model group. Expression of caspase-9 of DXQ middle dose group significantly decreased compared with model group. Expression of caspase-3 of DXQ low, middle, high dose group significantly decreased compared with model group. Conclusion ： These results suggest that DHQ has neuroprotective effects, which is related to the level of Apaf- 1, caspase-9 and easpase-3 restraining cells apoptosis.