积雪草苷立方液晶制备工艺与含量测定方法研究

Study of the Preparation Technology and Content Determination Method of the Cubic Liquid Crystalline of Asiaticoside

目的:考察积雪草苷立方液晶的制备工艺及含量测定方法。方法:前体-注入法制备积雪草苷立方液晶,用均匀设计筛选优化制备工艺,透析法分离游离药物,结合HPLC法,流动相:乙腈:2 mmol/Lβ-环糊精(24∶76),柱温25℃,检测波长205 nm,流速0.8 mL/min,进样量10μL,在此色谱条件下测积雪草苷包封率和载药量。结果:透析时间12 h,游离药物基本从积雪草苷立方液晶中分离,经均匀设计试验验证积雪草苷立方液晶的药物包封率可达39.8%。结论:前体-注入法制备积雪草苷立方液晶包封率高、简便可行,透析法结合HPLC测定药物含量准确。

Objective ： To establish the preparation technology and content determination method of the liquid crystalline of asiatieoside. Methods： Using precursor-injection method to prepare the liquid crystalline nanoparticles, uniform design to optimize the preparation process, dialysis method for separating free drug, and the HPLC method was adopted with the following condition： Agilent Cls column （ 4.5 mm×250mm,5μ m ）, the mobile phase was acetonitrile-2 mmol/L β -cyclodextrin （ 24 ： 76 ）with flow rate 0.8 mL/min, the detection wavelength was at 205 nm and the column temperature was 25 centigrade. Results ： Dialysis time was 12 h so that the free drug was separated from the liquid crystalline of asiaticoside, and test verification of liquid crystalline of asiatieoside about the drug encapsulation efficiency was accessible 39.8%. Conclusion ： The method of precursor-injection preparing the liquid crystalline of asiaticoside with good drug encapsulation efficiency is simple and feasible. The dialysis method combined with HPLC is used to determine the drug content accurately.