蒙古沙冬青AmMYB4-like基因的克隆与表达分析

Cloning and Expression Analysis of AmMYB4-like in Ammopiptanthus mongolicus

基于已有研究工作基础,从蒙古沙冬青[Ammopiptanthus mongolicus(Maxim)Cheng.f]中分离到1个编码MYB类转录因子基因,命名为Am MYB4-like。该序列长度为1 145 bp,含有一个由960 bp组成的开放阅读框,编码319个氨基酸,具有R2和R3保守结构域,属于R2R3-MYB转录因子。荧光定量PCR分析结果表明,Am MYB4-like在叶片中参与低温和干旱胁迫应答,在根中主要参与干旱胁迫应答。构建了p PZP212-Am MYB4-like植物表达载体,旨为进一步研究Am MYB4-like的功能奠定基础。

Based on our previous work, a gene encoding MYB transcription factor was cloned from Ammopiptanthus mongolicus, and it was designated as AmMYB4-1ike. The full length of the AmMYB4-like eDNA was 1, 145 bp, including a 960 bp opening reading frame （ ORF ） which encoded a 319-amino acid peptide. AmMYB4-like contained R2 and R3 conserved domains and belonged to the R2R3-MYB subfamily. Quantitative real-time PCR （ qRT-PCR ） analyses revealed that AmMYB4-like was up-regulated in leaves under low temperature and drought stresses, but it was mainly induced by drought stress in roots. Plant expression vector pPZP212- AmMYB4-like was constructed for further research of AmMYB4-like.