普伐他汀对烟熏大鼠肺部急性炎症抑制作用的初步研究

Effect of inhaled pravastatin on acute lung inflammation in cigarette smoking-exposed rat

目的探讨雾化吸入普伐他汀对烟熏大鼠肺部急性炎症的影响。方法 32只6~8周龄的SD大鼠,随机分为正常对照组、烟熏组、生理盐水组和普伐他汀钠组。正常对照组大鼠不予特殊处理,其它3组予以香烟烟雾暴露1周,生理盐水组、普伐他汀钠组分别在烟熏同时雾化吸入生理盐水或普伐他汀钠。1周后处死大鼠,比较各组大鼠的体重、肺泡灌洗液细胞数、血浆白介素(IL)-10、IL-17浓度及IL-10/IL-17的比值,以及肺组织中炎症的变化。结果接受烟熏处理的大鼠体重增加均有所减少,各组之间差异无统计学意义,烟熏组大鼠肺内有明显的急性炎症改变,肺泡灌洗液中炎症细胞数量及血浆中IL-10浓度明显增高,与正常对照组相比,差异有统计学意义(P<0.05)。雾化吸入普伐他汀钠组大鼠肺内炎症病理变化明显减轻,IL-10浓度增高较弱,IL-10/IL-17比值接近1。结论雾化吸入普伐他汀钠对烟熏导致的大鼠急性肺部炎症有一定的抑制作用。

Objective To investigate the effect of inhaled pravastatin on the acute inflammation of lung in smoked rats. Methods 32 male SD rats were randomly divided into four groups （n = 8 per group） , as the controls, ciga- rette-smoking exposure group, saline or pravastatin treatment groups. The rats in the control group were treated rou- tinely, the other groups were exposed to cigarette smoking for one week. Rats in saline or pravastatin group were treated with saline or pravastatin inhalation respectively. During smoking exposure period rats were weighted before and after the treatment, executed at day eight, blood, bronchoalveolar lavage fluid（BALF） , and lung tissue were collected. The morphological alternations of lung tissue were observed. Total cell numbers in BALF were counted. Enzyme-linked immunosorbent assay（ELISA） was used to detect the levels of serum IL-10 and IL-17 content. Re- sults The increase of body weight of smoke-exposed rats were less than that of the controls. In smoking-exposed rats, acute inflammatory changes were remarkable in the lung. The total cell numbers in BALF and levels of IL-10 were increased significantly（P 〈0. 05）. These changes were mitigated in pravastatin treated rats and an IL-10/IL- 17 rebalanee was observed. Conclusion Inhalation of pravastatin sodium has a certain inhibitory effect on cigarette smoke-induced acute lung inflammation in rats.