不同萃取方式分析武夷水仙茶挥发性成分

Analysis of volatile components in Wuyi narcissus tea by different extracted methods

分别采用同时蒸馏萃取(SDE)、静态顶空(SHS)和固相微萃取(SPME)三种不同香气萃取方式提取香气物质,进行气相色谱-质谱(GC-MS)分析,结合保留指数法对武夷水仙茶的挥发性物质进行了研究。三种萃取方法共鉴定出179种挥发性成分,其中SDE法121种、SPME法114种及SHS法61种。三种方法共有化合物有21种,SDE和SPME法共有化合物有72种,SHS和SDE共有化合物有25种,SHS和SPME共有化合物有37种。进一步分析已确定的挥发性成分,SDE比SPME多分离出醇类5种、烷烃类6种、酸类2种、酯类1种、酚类1种及醌类1种;SPME比SDE多分离出杂环类2种、醛类4种、芳香烃类1种及烯类2种。结果表明,SDE和SPME两种方法结合提取茶叶中的香气物质可以更全面,共鉴定出162种化合物。以花香、果香、木香、坚果香、烘焙香、焦糖香的香气物质为主。

The volatile components of Wuyi narcissus tea were extracted by simultaneous distillation extraction （SDE）, static headspace （ SHS）, solid- phase micro- extraction （ SPME ） and analyzed by GC- MS with retention index comparison. The results showed that 179 compounds were extracted by the three methods,including 121 compounds extracted by SDE, 114 compounds extracted by SPME and 61 compounds extracted by SHS.21 common compounds were extracted by three methods,72 common compounds were extracted by SDE and SPME,37 common compounds were extracted by SPME and SHS, and 25 common compounds were extracted by SDE and SHS.Compared with SPME methods,there were more identified compounds including 5 alcohols,6 hydrocarbons,2 acids, 1 phenol, 1 ester and 1 quinone, and less compounds including 2 heterocyclics,4 aldehydes, 1 aromatic hydrocarbons and 2 olefins compound extracted by SDE. The results showed that there were favorable for the identification of tea quality by the combined methods of SDE and SPME.One hundred and sixty-two volatile components were identified from Wuyi narcissus tea by the two methods.The smell of fruity ,floral ,woody, nutty, toasty, spicy caramelic were the typical ordor.