白芸豆α-淀粉酶抑制剂对α-淀粉酶抑制特性的研究

Study on the inhibitory properties ofα-amylase inhibitor from white kidney beans

本文研究了白芸豆α-淀粉酶抑制剂(α-AI)对α-淀粉酶的抑制特性。通过探究淀粉浓度、孵育时间、α-淀粉酶活力、淀粉类型对白芸豆α-AI抑制作用的影响来反映α-AI对α-淀粉酶的抑制特性。结果表明:白芸豆α-AI对α-淀粉酶的抑制作用属于非竞争性抑制,该抑制作用基本不受淀粉类型的影响,会随孵育时间的延长而显著增强(p<0.05),当α-淀粉酶活力过高(>1.125 U)时,抑制作用显著降低(p<0.05)。

The inhibitory properties of α- amylase inhibitor （ a- Al） from white kidney beans were analyzed by the effect of starch concentration,preincubation time,α-amylase activity and starch breed on the inhibition of α-Al.The results showed that the inhibition of α-Al on a-amylase was non-competitive and the variation of starch did not affect the inhibition.With the extension of preincuation time, the inhibition significantly increased （p 〈 0.05 ）.While with α- amylase activity exceeded 1.125 U, the inhibition significantly decreased（p 〈0.05 ）.