基于胞外多糖和菌丝生物量的香菇发酵培养基优化

Optimization of fermentation medium for extracellular polysaccharides and biomass by Lentinus edodes

为研究液体发酵培养基对香菇胞外多糖和菌丝生物量的影响,以秦巴山区香菇808菌株为试材,采用Plackett-Burman设计实验、最陡爬坡实验和响应曲面法对其液体发酵培养基的碳源、氮源和其它营养物质进行优化。结果表明,香菇胞外多糖发酵培养基的最佳组合是(g/500 m L):蔗糖7.18,玉米粉15.00,麦麸14.05,酵母膏0.35,KH2PO40.50,Mg SO4·7H2O 0.50,p H自然,胞外多糖实测值为0.967 g/500 m L;香菇菌丝生物量发酵培养基的最佳组合是(g/500 m L):蔗糖7.18,玉米粉15.00,麦麸14.05,酵母膏0.35,KH2PO40.75,Mg SO4·7H2O 0.50,p H自然,菌丝生物量实测值为28.146 g/500 m L。优化后的香菇胞外多糖产量和菌丝生物量较优化前分别提高12.44%和11.00%。此研究结果可为香菇液体发酵的中试生产提供理论依据。

Optimization of fermentation medium for extracellular polysaccharides （ EPS ） and biomass by Lentinus edodes was studied by the method with Plackett-Burman design, the steepest ascent experiment and Box-Behnken design.The Letinous edodes 808 was used as the test material, for study the effect of liquid fermentation medium on the extracellular polysaccharide and mycelial biomass of Lentinula edodes. The results showed that the best medium combination for EPS were （ g/500 mL） sucrose 7.18,corn meal 15.00, wheat bran 14.05, yeast extract 0.35, KH2PO4 0.50, MgSO4· 7H20 0.50, natural pH. In this condition, EPS yield was 0.967 g/500 mL.The best medium combination for biomass yield were（ g/500 mL）sucrose 7.18, corn meal 15.00, wheat bran 14.05, yeast extract 0.35, KH2 PO4 0.75, MgSO4 ·7H20 0.50, natural pH.In this condition, biomass yield was 28.146 g/500 mL.All of the EPS yield and the biomass yield in the fermentation medium increased 12.44% and 11.00% as compared with that in the initial medium. This results could provide a theoretical basis for fermentation pilot production of Lentinus edodes.