薏苡仁油β-环糊精包合物的制备及其表征

Preparation and characterization of Adlay seed oil and β-cyclodextrin inclusion complexes

采用饱和水溶液法制备薏苡仁油β-环糊精(β-CD)包合物;Box-Behnken响应面法优化并验证制备条件为:包合投料质量比(β-CD∶薏苡仁)为9∶1,包合温度45℃,包合时间6 h。通过SEM、DSC、FTIR、XRD表征薏苡仁油β-CD包合物的形成;HPLC法测定β-CD对薏苡仁油包合率达63%;包合物溶液以最大浓度(63 mg/m L)、最大给药量(0.04 m L/g)小鼠灌胃14 d后,心、肝、脾、肺、肾整体观察和各脏器切片均无明显病理变化,提示制备的薏苡仁油β-CD包合物食用安全,可进一步进行功效研究。

To prepare the β-cyclodextrin inclusion of Adlay seed oil with a saturated aqueous solution.The optimum by Box- Behnken response surface design were as follows-the ratio of β-CD to Adlay seed oil 9：1 （g/g） , stirring temperature 45 ℃ , inclusion time 6 h. The inclusion was characterized by SEM, DSC, FTIR and XRD. Entrapment efficiency of the inclusion determined by HPLC was 63%.In the acute toxicity test,the mice which were fed with the highest concentration （63 mg/mL） and maximum dosage（0.04 mL/g）, no death was found in the experimental mice after 14 days. No organ abnormalities were found and no organ pathology in sliced observation.The prepared inclusion complexes were safety for oral administration to be better for turther study of efficacy.