红枣浓缩汁缓解小鼠体力疲劳及调节免疫功能的研究

Effect of jujube juice concentrate on relieve physical fatigue and immune function in mice

目的:研究红枣浓缩汁缓解小鼠体力疲劳及调节免疫功能的作用。方法:实验设溶剂对照组(蒸馏水)和2.5、5.0、10.0 m L/kg·bw三个红枣浓缩汁组。选择运动耐力实验以及肝糖原、肌糖原、血乳酸、血清和肝脏尿素氮、肌肉总蛋白等相关生化指标,评价红枣浓缩汁对小鼠体力疲劳的影响;选择脾脏抗体生成细胞数、半数溶血值、迟发型变态反应实验、脾淋巴细胞转化实验和碳粒廓清实验等免疫学指标,分别从体液免疫、细胞免疫和非特异性免疫功能探讨红枣浓缩汁对小鼠免疫功能的影响。结果:与溶剂对照组相比,低剂量组运动耐量显著增加(p<0.05),血乳酸及尿素氮含量极显著降低(p<0.01),中剂量组肝糖原和肌糖原含量极显著提高(p<0.01),高剂量组肌肉的蛋白质含量显著提高(p<0.05)。免疫学研究表明,中剂量组红枣浓缩汁能显著提高小鼠吞噬指数和抗体生成细胞数(p<0.01),以及抗体积数(p<0.05),高剂量组红枣浓缩汁可显著提高小鼠的足趾厚度、抗体积数(p<0.01),以及吞噬指数和抗体生成细胞数(p<0.05)。结论:红枣浓缩汁具有缓解小鼠体力疲劳及增强免疫功能的作用。

Objective：To study the effect of jujube juice concentrate on anti-fatigue activity and immune function in mice. Method：Mice were randomly divided into 4 groups including solvent control group（ distilled water）and 2.5,5.0,10.0 mL/kg, bw. In order to evaluate the effect of jujube juice concentrate on anti-fatigue activity in mice ,the rod drop time were recorded, and the content of liver glycogen, muscle glycogen, blood lactic acid, blood urea nitrogen, total protein were tested by experiment. The plaque forming cell of spleen, phagocytic index, antibody level, the thickness of footput and spleen lymphocyte transformation were evaluated from two aspects of specific immunity and non- specific immunity. Results ： Compared to the solvent group,in low dose group, the anti-fatigue time significantly lengthen （p 〈 0.05 ）, the content of blood laetic acid and blood urea nitrogen significantly decreased （p 〈 0.05 ） , in middle dose group, the content of liver glycogen and muscle glycogenhighly significantly increased（p 〈 0.01 ） ,in high dose group, the content of muscle protein significantly increased（p 〈 0.05 ）.The middle dose could not only highly significantly improve the mouse plaque forming cell of spleen and phagocytic index（p 〈0.01 ） ,but also significantly improve the antibody level（p 〈0.05 ）.The high dose could not only highly significantly improve antibody level and thickness of footput（p 〈 0.01 ）, but also significantly improved mice plague forming cell of spleen and phagocytic index（p 〈 0.05 ）.Conclusion：Jujube juice concentrate had anti-fatigue effect and enhanced immune function of mice.