沈阳地区临床与环境分离的嗜肺军团菌的同源性

Homology of Legionella strains isolated from clinical cases and environmental water in Shenyang

目的分析沈阳地区临床与环境分离的嗜肺军团菌（LP）的同源性。方法收集2012年7月-2014年9月来自沈阳市2例LP感染患者、2家医院及13家公司生活用水和空调冷凝塔水中分离得到的22株LP。对菌株进行复苏并应用AscI内切酶进行脉冲场凝胶电泳，采用SPSS19．0软件对指纹图谱进行聚类分析。结果22株菌中20株为LPl型，其余2株分别为LP7型和LP8型；共分为14种基因型，其中SYC型有7株，同一血清型菌株可呈不同基因型；同一地点可分离出不同血清型和基因型的菌株；同一医院冷却塔和生活用水中分离的LP菌株有同源性。结论沈阳地区从临床及环境分离的LP具有基因多态性；同一医院不同水系中存在的LP菌株具有同源性。

Objective To study the homology of Legionella pneumophila （LP） strains which were isolated from clinical specimens and environmental water. Methods Twenty-two Legionella strains were collected in which 2 strains were isolated from 2 cases of LP infection patients, and others were isolated from domestic water or air conditioning cooling tower water of 2 hospitals and 13 companies from July 2012 to September 2014 in Shenyang. They were recovered and analyzed by the pulse field gel electrophoresis （PFGE） with Ase I restriction enzymes. Data were cluster analyzed using SPSS 19. 0. Results Twenty of 22 strains were LP1, and others were LP7 and LP8. Twenty-two strains could be divided into 14 genotypes, and 7 strains belonged to SYC banding pattern. The same bacterial serotype presented with different genotypes. Different bacterial serotypes and genotypes of LP strains can be found in the same place, and homology could be observed between LP strains isolated from cooling tower water and domestic water within the same hospital. Conclusion In Shenyang area, LP strains which are isolated from clinical cases and environmental water have the characteristics of gene polymorphism. Legionella strains isolated from different water system in the same hospital have homology.