摘要
目的 探究SIRT1基因沉默对DLBCL放射敏感性影响。方法 利用免疫组织化学观察SIRT1在DLBCL组织中的蛋白表达情况,蛋白印迹法检测SIRT1在DLBCL细胞系OCI-Ly3、SU-DHL-2、SU-DHL-4及人正常B细胞永生化细胞系HMy2.CIR中的表达情况。利用Lipofectamine 2000转染试剂盒将si-SIRT1及si-NC转染到SU-DHL-4细胞中并检测转染后SIRT1的表达;MTT法和克隆形成实验检测经放射处理后细胞放射敏感性的变化。成组t检验差异或单因素方差分析。结果 SIRT1在DLBCL组织中的阳性率为72.6%(103/140),明显高于SIRT1在RLH中的阳性率(26.5%,8/25)(P=0.001);SIRT1在DLBCL细胞系中的表达水平显著高于人正常B细胞永生化细胞系HMy2.CIR细胞(P=0.020);SIRT1基因沉默后,SIRT1在SU-DHL-4细胞中的表达量明显降低(P=0.008)。放射处理后,SIRT1基因沉默组的细胞增殖率和克隆形成率显著低于对照组(P=0.030)。结论 SIRT1基因沉默明显提高了DLBCL细胞的放射敏感性,为DLBCL细胞的治疗提供了一个新的靶标。
Objective To explore the effect of SIRT1 gene silencing on the radiosensitivity of diffuse large B-cell lymphoma (DLBCL) cells. Methods Immunohistochemistry was used to measure the protein expression of SIRT1 in DLBCL tissues. Western blot was used to measure the expression of SIRT1 in DLBCL cell lines (OCI-Ly3, SU-DHL-2, and SU-DHL-4) and the immortalized B cell line HMy2.CIR. After SU-DHL-4 cells were transfected with si-SIRT1 and si-NC using Lipofectamine 2000, the expression of SIRT1 was determined by Western blot. MTT assay and colony-forming assay were used to assess the cell growth and colony formation ability of SU-DHL-4 cells treated with radiation. The group t-test or univariate analysis of variance was used for comparison between groups. Results The expression rate of SIRT1 in DLBCL tissues was 72.6%(103/140), which was significantly higher than that in reactive lymphoid hyperplasia (RLH) tissues (26.5%, 8/25)(P=0.001). The SIRT1 expression was significantly higher in DLBCL cells than in HMy2.CIR cells (P=0.020). After SIRT1 gene silencing by si-SIRT1, the expression of SIRT1 was significantly reduced in SU-DHL-4 cells (P=0.008). Besides, SIRT1 gene silencing significantly reduced the growth rate and colony formation ability of SU-DHL-4 cells treated with radiation (P=0.030). Conclusions SIRT1 gene silencing enhances the radiosensitivity of DLBCL cells, providing a novel target for the radiotherapy of DLBCL.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2017年第6期687-690,共4页
Chinese Journal of Radiation Oncology