胎盘FAT/CD36表达与巨大儿发生的相关性

Correlation between FAT/CD36 expression in placenta and macrosomia

目的:探讨胎盘组织中脂肪酸转位酶(FAT/CD36)表达与巨大儿发生的关系。方法:选择2014年6月至2016年6月在温州医科大学附属第二医院育英儿童医院正常妊娠并足月分娩的89例产妇和新生儿为研究对象,其中44例为巨大儿,45例为正常儿。采用自制调查表收集产妇和新生儿的基本信息,并收集胎盘样品。采用实时荧光定量PCR(q PCR)技术及Western blot法检测巨大儿与正常儿胎盘组织中FAT/CD36基因和蛋白的表达水平,采用多因素logistic回归进行巨大儿发生的影响因素分析。结果:q PCR和Western blot结果显示,巨大儿组FAT/CD36表达水平高于正常儿(P<0.05)。多因素logistic回归分析表明,胎盘FAT/CD36基因m RNA高表达为巨大儿发生的危险因素,且与孕周、孕期体质量增加、新生儿性别共同影响巨大儿的发生。结论:FAT/CD36在胎盘组织中表达上调可能是导致巨大儿发生的机制之一。

Objective： To explore the correlation between expression of FAT/CD36 in placenta and macrosomia. Methods： The cases were collected from June 2014 to June 2016 in the Second Affiliated Hospital ＆ Yuying Children＇s Hospital of Wenzhou Medical University, including 44 macrosomia newborns and 45 normal birth weight newborns. A self-designed questionnaire was used to collect related information of pregnant women and their neonates, real-time fluorescent quantitative PCR （qPCR） was used to detect FAT/CD36 mRNA expression in placenta. Western blot was used to detect FAT/CD36 protein expression. The influencing factors of macrosomia were analyzed by multivariate logistic regression model. Results： qPCR and Western blot showed FAT/CD36 expression of macrosomia was significantly higher than that of healthy controls （P〈0.05）. The result of multivariate logistic regression analysis showed that high expression of FAT/CD36 mRNA expression was a risk factor, and the occurrence of macrosomia was influenced by gestational age, gestational weight gain and newborns sex. Conclusion： The up-regulation of FAT/CD36 in placenta may be correlated with macrosomia.