金丝桃苷对小鼠T淋巴细胞亚群及血清细胞因子的影响

Effects of Hyperoside on T Cell Subset and Serum Cytokines in Mice

观察金丝桃苷对小鼠免疫系统的作用,将Balb/c小鼠随机分成5组,空白对照组(在第1、3、5天腹腔注射生理盐水)、免疫抑制组(在第1、3、5天腹腔注射环磷酰胺)、给药组(在第1、3、5天腹腔注射环磷酰胺,第1~7天每天以150/300mg/kg金丝桃苷水溶液灌胃)和阳性对照组(在第1、3、5天腹腔注射环磷酰胺,第1~7天每天以0.2mg/kg黄芪多糖水溶液灌胃)。用ELISA法测定小鼠T淋巴细胞亚群CD_3、CD_4、CD_8以及细胞因子IL-4、IL-6、IL-10、IFN-γ、TNF-α的水平。结果表明,与空白对照组相比,免疫抑制组的CD_3、CD_4、CD_8水平以及血清细胞因子IFN-γ、TNF-α水平显著降低(P<0.05),IL-4、IL-6及IL-10水平显著升高(P<0.05)。给予金丝桃苷后第8天,与免疫抑制组相比,CD_3、CD_4、CD_8水平以及血清细胞因子IFN-γ、TNF-α水平极显著升高(P<0.01),IL-4、IL-6、IL-10水平极显著降低(P<0.01)。金丝桃苷能通过提高免疫抑制小鼠T淋巴细胞亚群的CD_3、CD_4、CD_8水平以及细胞因子IFN-γ及TNF-α水平,降低IL-4、IL-6、IL-10水平,来拮抗环磷酰胺所致的免疫机能抑制,提高机体免疫功能。

To investigate the effects of hyperoside on the immune system of mice,the Balb/c mice were randomly divided into 5 groups, blank control group （intraperitoneal injection of saline on days 1,3 and 5）,immunosuppressed group （intraperitoneal injection of cyclophosphamide on days 1,3 and 5）,drug group （intraperitoneal injection of cyclophosphamide on days 1,3 and 5; gavage administration of 150/300 mg/kg hyperoside on days 1-7）,positive control group （intraperitoneal injection of cyclophosphamide on days 1,3 and 5 ,gavage administration of 0.2 mg/kg astragalus polysaccharide on days 1-7）.Then the levels of T cell subset （CD3 ,CD4, CD8 ） and five serum cytokines （IL-4, IL-6, IL-10, IFN-7, TNF-α） were measured by ELISA. The expressions of CD3, CD4 , CD8, IFN-）,, and TNF-a decreased significantly and the levels of IL-4, IL-6 and IL-10 increased significantly compared with blank control group.After treatment with hyperoside, the levels of CD3, CD4, CDs, IFN-7 and TNF-α increased very significantly, while the levels of IL-4, IL-6, and IL-10 reduced very significantly compared with the immunosuppressed group.Hyperoside can improve the immune function by increasing the levels of CD3, CD4, CDs, IFN-γ and TNF-α, and decreasing the levels of IL-4,IL-6 and IL-10 in immunosuppressed mice.