不同胚胎冷冻保存时间和方法对FET周期临床结局的影响

Study on clinical outcome of frozen-thawed transfer cycles with different cryopreservation duration of programmed freezing and vitrification embryos

目的:比较分析玻璃化和程序化冷冻卵裂期和囊胚期胚胎保存后复苏效果及临床结局。方法:根据不同冷冻保存时间将胚胎冻融周期分为4组,分别为1~6个月组、7~12个月组、13~24个月组和≥25个月组,比较四组的复苏率、临床妊娠率和流产率。根据不同冷冻保存方法分为玻璃化冷冻组和程序化冷冻组。结果:4组胚胎复苏存活率(98.1%、99.0%、96.8%和95.7%)、临床妊娠率(54.4%、55.4%、49.6%和51.5%)和流产率(10.7%、8.9%、9.45和11.15)相比较,差异均无统计学意义(P>0.05)。玻璃化冷冻组胚胎复苏率和临床妊娠率均显著高于程序化冷冻组(P<0.05)。结论:胚胎冷冻保存时间不影响解冻后胚胎质量。玻璃化冷冻胚胎方法复苏效果优于程序化冷冻法。囊胚期冻融胚胎可获得较高的临床妊娠率。

Objective To compare the influence of eryopreservation duration on quality of rozen-thawed embryo and clinical outcome in frozen-thawed transfer cycles. Methods A retrospective study was performed to evaluate the clinical outcomes and quality of rozen-thawed embryo of frozen embryo transfer （FET） cycles includ- ing freezing cycles and vitrification cycles. FET cycles were divided into 4 group aeorrding to the storage duration of vitrified embryos ： 1 - 6 months group, 7 -12 months group, 13 - 24 months group and ≥ 25 months group. Result There was no significant difference in recovery rate, clinical pregnancy rate and abortion rate （98.1%, 99.0%, 96.8% ＆ 95.7% ; 54.4%, 55.4%, 49.6% ＆ 51.5% ; 0.7%, 8.9%, 9.4% ＆ 11.1% ; P 〉 0.05 ）. The embryos recovery rate and clinical pregnant rate after vitrification were significantly higher than programmed freezing （P 〈 0.05）. The blastocyst recovery rate in group A was significantly higher than the cleavage stage embryo recovery rate in group B （P 〈 0.05）. Conclusions Vitrification has better effects on eryopreservation and clinical outcomes. Blastoeyst may be the best stage for vitrifying and freezing of embryo.