苯并芘激活ERK1/2信号通路促进气道平滑肌细胞外基质蛋白沉积对气道重塑的影响

Benzopyrene activates ERK1/2 signal pathway to promote extracellular matrix protein deposition of airway smooth muscle cells on airway remodeling

目的探讨苯并芘对人气道平滑肌细胞(HASMC)细胞外基质(ECM)蛋白沉积的影响及相关通路机制。方法原代培养HASMC,将2~6代细胞用于实验。用实时荧光定量PCR和Western Blot法分别检测ECM的基因及蛋白的表达量;用Western Blot法分析ERK1/2的磷酸化水平。结果苯并芘可以诱导HASMC胶原蛋白Ⅰα1(P<0.01)及ECM蛋白(包括胶原蛋白Ⅰα1、多功能蛋白聚糖、纤连蛋白、层粘连蛋白α2)mRNA表达增加(P<0.05)。苯并芘可引起ERK1/2磷酸化水平快速增高(P<0.01);ERK通路抑制剂PD98059能显著抑制苯并芘诱导的胶原蛋白Ⅰα1(P<0.01)及ECM蛋白(包括胶原蛋白Ⅰα1、纤连蛋白、多功能蛋白聚糖、层粘连蛋白α2)mRNA表达增加(P<0.01)。结论苯并芘通过激活ERK1/2通路诱导HASMC中ECM蛋白沉积,阻断ERK1/2信号通路可以抑制苯并芘诱导的气道重塑。

Objeetive To investigate the influence of benzopyrene on extracellular matrix（ECM） protein deposition of human airway＇smooth muscle cells（HASMC） and the related pathway mechanism. Methods HASMC were primarily cultured and the 2-- 6 generations cells were applied in this experiment. The expression amount of ECM gene and protein was detected by real time PCR and Western Blot the phosphorylation level was analyzed by using the Western Blot method, Results Benzopyrene could to increase the expression of H ASMC collagen I α1 protein （P〈0.01） and ECM protein （including collagen I α1, versican, fibronectin, laminin a2） mRNA（P〈：0.05）. Benzopyrene could induce the rapid increase of ERK1/2 phosphorylation level （P〈0.01）. Furthermore,the ERK pathway inhibitor PD98059 could significantly inhibit the increase of benzopyrene-induced collagen I α1 （P〈 0.01）and ECM protein（including collagen I α1,versican, fibronectin, laminin a2） mRNA expression（P〈 0.01）. Conclusion Benzopyrene induces the ECM protein deposition of HASMC by activating the ERK1/2 pathway, blocking the ERK1/2 signal pathway can inhibit the benzopyrene-induced airway remodeling,