人软骨TNF-α、MMP13和关节液IL-1β在两种湿证膝关节炎中的表达

Expression of TNF-α, IL-1β and MMP13 in Osteoarthritis Tissue of Type Two of Damp Syndromes

目的:探讨湿热证和寒湿证膝关节炎患者关节软骨中TNF-α、MMP13表达的异同,以及膝关节液中IL-1β含量的差别。方法:符合纳入标准的62例膝关节炎患者,分湿热型膝关节炎32例和寒湿型膝关节炎30例,术前抽取关节液,在膝关节置换术过程中,留取软骨标本,然后对其检测。结果:湿热组关节软骨中TNF-α的表达和寒湿组比较,差异具有统计学意义(P<0.05);MMP13的表达和湿热组比较,差异具有统计学意义(P<0.01);湿热组关节液中IL-1β的含量和寒湿组比较,差异具有统计学意义(P<0.05)。结论:湿热组中关节软骨炎症表达高于寒湿组,软骨破坏程度较寒湿组严重。

Objective： In order to observe the expression of TNF- α , MMP13 in cartilage of osteoarthritis of type of damp-heat and cold-dampness syndromes, and to observe content of IL-1 β in knee joint fluid. Method： 62 cases of osteoarthritis were divided into two groups： damp-heat group： 32 cases of type of damp-heat syndromes and cold dampness group： 30 cases of type of cold-dampness syndromes. Extract the joint fluid before the operation, and take the sample of knee cartilage during the operation, then test the sample. Results： To the cold-dampness group, there was significant difference in expression of TNF-α and content of IL-1β （P〈0.05）. To the damp-heat group, there was sig- nificant difference in expression of MMP13 （P〈0.01）. Conclusion： The expression of TNF- α and content of IL-1 β of damp-heat group were higher than the cold-dampness group＇ s, and the expression of MMP13 of damp-heat group was lower than the cold-dampness group＇ s.