石榴皮鞣质改善慢性肾小球肾炎大鼠的尿液代谢组学的通路分析

Analysis of Urinary Metabonomics Pathway in Rats with Chronic Glomerulonephritis Intervened by Tannins from Pericarpium Granati

目的:探讨石榴皮鞣质改善慢性肾小球肾炎大鼠尿液代谢组学的通路。方法:运用KEGG数据库进行信号通路分析,用HMDB数据库对代谢产物分子注释、相关的酶或转运蛋白及其相关性质进行分析,Met PA网络软件对代谢产物路径进行可视化作图。结果:12个差异性代谢产物共参与了16条代谢途径,其中"色氨酸代谢"、"三羧酸循环"、"苯丙氨酸代谢"、"半胱氨酸和甲硫氨酸代谢"的代谢通路影响显著(P<0.05)。结论:石榴皮鞣质干预下的慢性肾小球肾炎大鼠中"色氨酸代谢"、"三羧酸循环"、"苯丙氨酸代谢"、"半胱氨酸和甲硫氨酸代谢"发生变化参与其病理过程。

Objective： To analyze the metabolicomics pathway in the rats with chronic glomerulonephritis intervened by tannins from Pericarpium Granati. Methods： Signal pathway analysis was carried out using the KEGG database and molecular metabolite anno- tation was performed using HMDB database. The enzyme or transporter and its related properties were analyzed. The metaholite path visualization was carried out by using MetPA network software. Results ： The analysis of biological metabolism pathway showed that 12 metabolites involved in 16 metabolic pathways. The pathway of tryptophan metabolism, citrate cycle, phenylalanine metabolism, cyste- ine and methionine metabolism showed notable changes （ P 〈 0.05 ）. Conclusion： The changes of phenylalanine metabolism, citrate cycle, tryptophan metabolism, cysteine and methionine metabolism in the rats with chronic glomerulonephritis intervened by tannins from Pericarpium Granati participate the pathological process.