HPLC法同时测定玉真散中天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素的含量

Simultaneous Determination of Gastrodin,Prim-O-glucosylcimifugin,5-O-Methylvisammioside,Imperatorin and Isoimperatorin in Yuzhen Powder by HPLC

目的:建立HPLC法同时测定玉真散中天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素含量。方法:色谱柱为Phenomenex Gemini C_(18)柱(250 mm×4.6 mm,5μm),柱温为25℃,流速为1.0 ml·min^(-1),流动相为甲醇-水梯度洗脱,检测波长为230 nm,进样量为5μl。结果:天麻素在2.68~214.00μg·ml^(-1)范围内线性关系良好(r=0.999 9),平均回收率为100.2%,RSD为0.9%(n=6);升麻素苷在5.22~418.00μg·ml^(-1)范围内线性关系良好(r=0.999 7),平均回收率为99.9%,RSD为0.9%(n=6);5-O-甲基维斯阿米醇苷在4.57~365.80μg·ml^(-1)范围内线性关系良好(r=0.999 5),平均回收率为99.7%,RSD为1.0%(n=6);欧前胡素在5.22~417.20μg·ml^(-1)范围内线性关系良好(r=0.999 6),平均回收率为99.0%,RSD为0.9%(n=6);异欧前胡素在5.29~423.20μg·ml^(-1)范围内线性关系良好(r=0.999 8),平均回收率为100.2%,RSD为0.8%(n=6)。结论:该方法简单、准确,可同时测定5种成分的含量,可用于玉真散的质量控制。

Objective： To establish an HPLC method for the determination of gastrodin, prim-O-glucosylcimifugin, 5-O-methyl- visammioside, imperatorin and isoimperatorin in Yuzhen powder. Methods： A Phenomenex Gemini Cls column （250 mm x 4.6 mm, 5 μm） was used and the column temperature was 25 ℃. The flow rate was 1.0 ml · min - 1 and the mobile phase consisted of methanol- water with gradient elution. The detection wavelength was 230 nm and the sample size was 5 p,1. Results： The linear range of gastrodin was 2.68-214.0 μg · ml-l （r =0. 999 9）, and the average recovery was 100.2% （RSD =0.9%, n =6） ;the linear range of prim-O- glucosylcimifugin was 5.22-418.0 μg · ml-l （ r = 0.999 7 ）, and the average recovery was 99.9% （ RSD = 0.9%, n = 6） ; the linear range of 5-O-methylvisammioside was 4.57-365.8 μg · ml-l （ r = 0.999 5 ）, and the average recovery was 99.7% （ RSD = 1.0%, n = 6） ;the linear range of imperatorin was 5.22-417.2 μg · ml-l （r =0. 999 6）, and the average recovery was 99.0% （RSD =0.9%, n = 6） ; the linear range of isoimperatorin was 5.29-423.2 μg · ml-l（ r = 0.999 8 ）, and the average recovery was 100.2% （ RSD = 0.8%, n = 6）. Conclusion： The method is simple and accurate, and the contents of the five ingredients can be determined simultane- ously. The method can be used for the quality control of Yuzhen powder.