创伤性颅脑伤后小鼠皮层和海马Wnt3a和β-Catenin的表达变化

Changes in expression of Wnt3a and β-catenin in the cortex and the hippocampus after traumatic brain injury in mice

目的探讨创伤性脑损伤后小鼠皮层和海马Wnt3a和β-Catenin的表达及其意义。方法在小鼠创伤性脑损伤模型基础上,采用免疫印迹法和免疫组织化学法检测创伤性脑损伤后Wnt3a和β-Catenin的蛋白变化。结果 Wnt3a和β-Catenin在对照组的脑组织中表达较低。创伤性脑损伤后1 h,皮层和海马区β-Catenin和Wnt3 a的含量开始增加。与对照组相比,伤后6h和第7天,β-Catenin和Wnt3a蛋白表达显著增加(P<0.05)。免疫荧光染色结果显示,脑损伤24 h后,β-Catenin和Wnt3a在小鼠皮层和海马表达量均增加。结论小鼠创伤性脑损伤后早期和恢复期,Wnt3a和β-Catenin的蛋白水平在皮层和海马显著增高,这提示Wnt3a和β-Catenin可能在创伤性脑损伤的病理生理机制中发挥着重要的作用。

Objective To investigate the expression of Wnt3a and β-catenin in the cortex and the hippocampus after traumatic brain injury in mice and its clinical significance.Methods A mouse model of traumatic brain injury was established,and Western blotting and immunohistochemistry were used to measure the changes in the protein expression of Wnt3a and β-catenin after traumatic brain injury.Results The control group had low expression of Wnt3a and β-catenin in brain tissue.At 1 hour after traumatic brain injury,the content of β-catenin and Wnt3a in the cortex and the hippocampus started to increase.At 6 hours and 7 days after traumatic brain injury,the traumatic group had significant increases in the protein expression of β-catenin and Wnt3a （P 〈 0.05）.Immunofluorescent staining showed that at 24 hours after brain injury,there were increases in the expression of β-catenin and Wnt3a in the cortex and the hippocampus.Conclusions The protein expression of Wnt3a and β-catenin increases significantly in the early stage and recovery stage after traumatic brain injury,suggesting that Wnt3a and β-catenin may play important roles in the pathophysiological mechanism of traumatic brain injury.