驽药针刺对大鼠脊髓损伤后运动功能及BDNF表达的影响

Effect of crossbow medicine needle therapy on motor function and expression of brain-derived neurotrophic factor after spinal cord injury in rats

目的研究驽药针刺在大鼠脊髓损伤后运动功能变化以及BDNF表达的变化。方法采用脊髓半横断损伤模型。100只SD大鼠随机分为对照组、假手术组、脊髓损伤组、单纯针刺组、驽药针刺组,每组分为3天、7天、14天、21天共4个亚组,每组5只。BBB法评定大鼠后肢运动功能变化,免疫组化法检测大鼠脊髓中BDNF的表达变化。结果 BBB评分显示驽药针刺组的各时间点评分均高于脊髓损伤组(P<0.05),驽药针刺组7、14、21d的BDNF表达均高于脊髓损伤组(P<0.05),且与BBB评分呈正相关(r=0.717,P<0.05)。结论驽药针刺可明显改善脊髓损伤大鼠的运动功能,并可明显促进大鼠脊髓损伤后BDNF的表达。

Objective To investigate the effect of crossbow medicine needle therapy on motor function and expression of brain-derived neurotrophic factor （BDNF） after spinal cord injury in rats.Methods A rat model of hemisection spinal cord injury was established.A total of 100 Sprague-Dawley rats were randomly divided into control group,sham-operation group,spinal cord injury group,simple acupuncture group,and crossbow medicine needle therapy group,and each group was further divided into 3-,7-,14-,and 21-day subgroups,with 5 rats in each subgroup.The BBB method was used to evaluate the changes in hindlimb motor function,and Immunohistochemistry was used to measure the changes in the expression of BDNF in the spinal cord.Results Compared with the spinal cord injury group,the crossbow medicine needle therapy group had a significantly higher BBB score at all time points （P 〈 0.05） and significantly higher expression of BDNF at 7,14,and 21 days （P 〈0.05）,which was positively correlated with BBB score （r =0.717,P 〈0.05）.Conclusions Crossbow medicine needle therapy can significantly improve motor function and promote the expression of BDNF after spinal cord injury in rats.