腺苷对大鼠中枢神经系统氧中毒所致急性肺损伤的保护作用

Studies on the protective effects of adenosine in acute lung injury induced by central nervous system oxygen toxicity

目的探讨腺苷对高压氧（hyperbaric oxygen, HBO）导致大鼠中枢神经系统氧中毒所致急性肺损伤保护作用。方法采用数字表法将24只SD大鼠随机分为正常组、对照组、腺苷组，每组8只。正常组大鼠不作任何处理，对照组及腺苷组大鼠经侧脑室分别注射生理盐水和腺苷，然后暴露于0.6 MPa HBO中，持续30 min。暴露结束后观察大鼠肺表面出血情况，取其肺泡灌洗液测定蛋白含量，取其肺组织进行含水量检测和组织病理学检查。 结果腺苷组大鼠肺表面出血损伤较对照组明显减轻，光镜下显示腺苷组大鼠肺组织炎细胞浸润、出血、水肿程度均有所降低。此外腺苷组大鼠肺泡灌洗液蛋白含量[（210±190） mg/L]比对照组[（730±230） mg/L]降低，对照组与正常组[（100±20） mg/L]相比蛋白含量升高，差异均有统计学意义（P〈0.05）；腺苷组肺组织含水量[（80.42±1.05） %]比对照组[（86.60±1.80） %]降低，差异有统计学意义（P〈0.05），对照组与正常组[（76.25±0.07） %]比较含水量也有一定升高，但差异无统计学意义（P〉0.05）。结论腺苷可以明显减轻大鼠中枢神经系统氧中毒引起的急性肺损伤。

Objective To investigate the protective effects of adenosine in acute lung injury induced by central nervous system （CNS） oxygen toxicity in the rats exposed to hyperbaric oxygen （HBO） and to further confirm the effects of adenosine against CNS oxygen toxicity. MethodsThe rats were randomly divided into 3 groups： the blank control group, the solvent-control group and the adenosine group. The blank control group didn＇t receive any treatment, while the solvent-control group and the adenosine group were respectively treated with normal physiological saline and adenosine（160 μg）. Then, the animals were exposed to HBO at a dosage of 0.6 MPa for 30 minutes and observations were made on the hemorrhage on the surface of the lungs. Samples of bronchoalveolar lavage fluid （BALF） were collected for protein level detection, and lung tissues were collected for water level and pathological detection.ResultsPulmonary surface bleeding of the adenosine group was significantly alleviated as compared with that of the vehicle-control group. Microscopic detection displayed that inflammatory cell infiltration, hemorrhage and pulmonary edema of the adenosine group were all decreased to some extent. In addition, BALF protein level of the adenosine group was also significantly decreased [（0.21±0.19） mg/L], as compared with that of the solvent-control group [（0.73±0.23） mg/L]. The protein level of the solvent-control group was also significantly increased, as compared with that of the blank control group [（0.10±0.02） mg/L], with statistical significance（P〈0.05）. Pulmonary water level of the adenosine group [（80.42±1.05） %] was significantly lower than that of the solvent-control group, also with statistical significance（P〈0.05）. Pulmonary water level of the solvent-control group was also increased to some extent, as compared with that of the control group [（76.25±0.07） %], but without statistical significance（P〉0.05）.ConclusionsResults indicated that adenosine could significantly alleviate acute pulmonary injury induced by CNS oxygen toxicity, which further demonstrated that adenosine had certain protective effects on CNS oxygen toxicity.