MT3基因启动子甲基化和肿瘤易感性与预后的相关性

Correlation association between MT3 gene promoter methylation and cancer susceptibility and the prognosis

目的探讨MT3基因启动子甲基化和肿瘤易感性与预后的相关性。方法选取2013年1月至2016年12月间邢台市人民医院收治的112例肿瘤患者,根据是否发生MT3基因甲基化分为研究组(发生基因启动子甲基化)23例与对照组(未发生基因启动子甲基化)89例。统计两组患者后代发生同样肿瘤的情况,对患者的近期效果进行评价,分析T3基因启动子甲基化和肿瘤易感性与预后的相关性,检测细胞周期和细胞凋亡率。结果研究组患者后代发生肿瘤比例显著高于对照组患者,差异有统计学意义(P<0.05)。对照组G0/G1期细胞比例显著高于研究组,S期的细胞比例显著少于研究组,凋亡率显著高于研究组,差异均有统计学意义(均P<0.05)。研究组患者治疗有效率为78.3%,对照组患者为93.3%,两组比较,差异有统计学意义(P<0.05)。MT3基因启动子甲基化与肿瘤易感性呈正相关,与预后状况、癌细胞周期和凋亡率呈负相关,差异有统计学意义(P<0.05)。结论 MT3基因启动子甲基化和肿瘤易感性呈正相关,与患者预后情况呈负相关。

Objective To analyze the relationship between methylation of MT3 gene promoter and cancer susceptibility and prognosis. Methods One hundred and twelve cancer patients were selected at Xingtai People＇s Hospital from January 2013 to December 2016. According to whether MT3 gene had methy- lation or not, patients were divided into a study group （with gene promoter methylation, 23 patients） and a control group （without methylation, 89 patients）. Incidence of the same cancer was calculated in offspring of the patients in the two groups. The recent curative efficacy was evaluated. The correlation between T3 gene promoter methylation and cancer susceptibility and prognosis was analyzed. The cell cycle and apopto- sis rate were detected by flow cytometry. Results Incidence of the same cancer in the offspring was signifi- cantly higher in the study group than in the control group （ P 〈 0. 05 ）. The proportion of G0/G1 cells was significantly higher, the proportion of calls in S phase was significantly less and the apoptosis rate was significantly higher in the control group than in the study group （P 〈0. 05）. The efficacy rate was 78.3% for the study group and 93.3% for the control group （P 〈 0. 05 ）. Methylation of MT3 gene promoter was positively correlated with cancer susceptibility, and negatively correlated with prognosis, cell cycle and apoptosis （P 〈 0.05 ）. Conclusion Methylation of MT3 gene promoter is positively correlated with cancer suscepti- bility, and is inversely correlated with the prognosis of patients.