异丙酚对宫内窘迫胎鼠的神经保护作用

Neuroprotective effect of propofol on fetal rat brain in intrauterine ischemia/reperfusion injury

目的观察异丙酚对宫内窘迫胎鼠的神经保护作用。方法 SD大鼠♀18只,交配后获得孕鼠。将孕鼠随机分为6组(n=3):假手术组(S组)、缺血/再灌注组(IR组)、异丙酚低、中、高剂量组(P1~P3组)和荷包牡丹碱组(B组)。S组和IR组孕鼠经尾静脉注射生理盐水1 m L。P_1~P_3组孕鼠经尾静脉分别注射异丙酚10、30、50 mg·kg^(-1)。B组在注射异丙酚50 mg·kg^(-1)的同时,腹腔注射荷包牡丹碱5 mg·kg^(-1)。夹闭子宫卵巢动脉11 min。再灌注3 d后取材,每只孕鼠各取2只胎鼠,每组6只。制作切片,计算海马CA1区神经元损伤指数(LI)。硫代巴比妥酸反应法检测胎鼠脑组织丙二醛(MDA)含量。结果 S组LI为7.2±0.9,MDA为(3.86±0.20)μmol·g^(-1),与之相比,IR组的LI为71.9±2.8,MDA为(9.10±0.45)μmol·g^(-1)(P<0.01)。与IR相比,异丙酚各组的LI分别为40.8±2.6,21.4±1.4,20.1±1.3,MDA分别为(7.32±0.41),(5.65±0.27),(5.44±0.28)μmol·g^(-1)(P<0.05)。B组的LI,MDA分别为(51.2±2.3),(7.54±0.31)μmol·g^(-1),翻转了异丙酚的保护作用。结论异丙酚对宫内窘迫胎鼠具有神经保护作用。

Aim To observe the neuroprotective effect of different doses of propofol on ischemic fetal ratbrain. Methods Eighteen healthy pregnant SD rats were randomly allocated into the following six groups with three rats in each. Group S: sham operation group,Group IR: ischemia/reperfusion group,Group P_1~ P_3: different doses of propofol groups,Group B:bicuculline group. In group S and group IR,1 ml saline solution was administered via caudal vein. In group P_1~ P_3,10,30,50 mg·kg^(-1)of propofol was administered via caudal vein respectively. In group B,when 50 mg·kg^(-1)propfol was administered via caudal vein,5 mg·kg^(-1)bicuculline was injected intraperitoneally at the same time. Bilateral uterine ovarian arteries were clamped for 11 mins to make intrauterine distress model of the fetal rats. The brains of fetal rats were removed after 3 days of reperfusion. Brain sections( 5 μm thick) were mounted and stained with Hematoxylin and eosin( HE). The profile of the hippocampus CA1 was evaluated under a light microscope and neuronal Lesion-index( LI) was calculated. MDA content of fetal rat brain was detected by thiobarbituricacid reaction method to determine the lipid peroxidation degree of brain. Results LI was( 7. 2 ± 0. 9) and MDA was( 3. 86 ± 0. 20) μmol · g^(-1) in group S. LI was 71. 9 ± 2. 8 and the content of MDA was( 9. 10 ±0. 45) μmol·g^(-1) in group IR,which increased significantly compared with those in group S( P < 0. 01). LI was( 40. 8 ± 2. 6),( 21. 4 ± 1. 4),( 20. 1 ± 1. 3) and the content of MDA was( 7. 32 ± 0. 41),( 5. 65 ±0. 27),( 5. 44 ± 0. 28) μmol · g^(-1) in propofol groups,which decreased significantly compared with those in group IR( P < 0. 05). LI and the content of MDA was( 51. 2 ± 2. 3),( 7. 54 ± 0. 31) μmol·g^(-1) in group B,respectively,reversing partly the neuroprotevtive effect of propofl. Conclusion Propofol could protect the neurons in hippocampus CA1 region of fetal rat against intrauterine distress by reducing the concentration of MDA in the brain.