胸苷激酶1在胸腔积液患者中的临床价值

Clinical significance of TK1 expressions in pleural effusions

目的探讨胸苷激酶1(Thymidine Kinase 1,TK1)在胸腔积液患者中的表达及临床诊断价值。方法将167例胸腔积液(Pleural Effusion,PE)患者按病理组织学分为恶性胸腔积液组(MPE)和良性积液组(BPE)。其中BPE组又包括结核性胸腔积液组(TPE)及肺炎旁胸腔积液组(PPE)。应用免疫印迹-增强化学发光法检测各组血清及积液TK1的水平,与其他血清和胸腔积液肿瘤标志物指标比较,并检测其在不同病理类型胸腔积液的水平。结果血清和积液TK1测定水平MPE组明显高于BPE组;在鉴别不同病理组织中TK1浓度则无显著差异。结论血清和积液中的TK1可作为鉴别良恶性胸腔积液的重要指标之一。

Objective To evaluate the relation and value of thymidine kinase （ TK1 ） in patients with dis-tinct etiologies of pleural effusions. Methods 167 patients with pleural effusion were divided into the malignant pleural effusion group （MPE） and the benign pleural effusion group （ BPE） according to pathological histology, and the BPE group included tuberculous pleural effusion （TPE） and parapneumonic effusion（ PPE）. The samples of TK1 were tested by chemoluminescent dot blot assay of serum and pleural effusion, and compared with other conventional biomarkers. Results The levels of sTKl and pTKl in malignant pleural effusion were obviously higher than those in the benign group （P 〈 0. 05 ） . In the diagnosis of different pathological of malignant pleural effusion, TK1 had no corresponding advantages. Conclusion TK1 in serum and fluid can be used in the diagnosis of malignant pleural effusion with high sensitivity.