人辅酶Ⅱ依赖性视黄醇脱氢/还原酶在新疆汉族妇女宫颈病变中的表达及意义

Expressions of NADP(H)-dependent retinol dehydrogenase/reductase in different lesions cervical tissues in Han women

目的:探讨人辅酶Ⅱ依赖性视黄醇脱氢/还原酶在新疆汉族妇女宫颈病变中的表达及临床意义,为新疆地区宫颈癌高危人群的识别、预防及诊断提供依据。方法:依据病理诊断结果,将80例新疆地区汉族妇女宫颈组织标本分为4组,正常及慢性炎症组20例、CINⅠ组20例、CINⅡ-Ⅲ组20例、宫颈鳞癌组20例,采用免疫组化SP法检测不同级别宫颈病变中DHRS4基因的表达。结果:DHRS4基因在正常及慢性炎症组、CINⅠ组、CINⅡ-Ⅲ组、宫颈鳞癌组标本中阳性表达有统计学意义(P<0.05),分别进行组间比较,正常宫颈及慢性炎症组与宫颈CINⅠ组有统计学差异(P<0.05),正常宫颈及慢性炎症组与宫颈CINⅡ-Ⅲ组有统计学差异(P<0.05),正常宫颈及慢性炎症组与宫颈癌组有统计学差异(P<0.05),宫颈CINⅠ组与宫颈癌组无统计学差异(P>0.05),CINⅡ-Ⅲ组与宫颈癌组无统计学差异(P>0.05)。结论:DHRS4基因在人体宫颈鳞癌组织中高表达,且随着宫颈病变的进展表达阳性率逐渐升高,可能与宫颈疾病及其癌变的发生有关。

Objective: To investigate the expression and clinical significance of NADP( H)-dependent retinol dehydrogenase/reductase in cervical lesions in Xinjiang Han women,and to provide evidence for the identification,prevention and diagnosis of cervical cancer at high risk population. Methods: According to the pathological diagnosis,the deta of 80 cases Xinjiang Han women were divided into four groups:Normal and chronic inflammatory group,CINⅠgroup,CIN Ⅱ-Ⅲ group,and cervical squamous cell carcinoma group,each group were 20 cases. Detecting the expressions of DHRS4 gene in cervical lesions by using immunohistochemistry SP method. Results:The DHRS4 gene in normal and chronic inflammatory group,CINⅠgroup,CIN Ⅱ-Ⅲ group,cervical squamous cell carcinoma group of cervical samples positive expression was significant difference( P < 0. 05),compared to each group,the normal and chronic inflammatory cervical group and CINⅠgroup were significant difference( P < 0. 05),the normal and chronic inflammatory cervical group and CIN Ⅱ-Ⅲ group were significant difference( P < 0. 05),the normal and chronic inflammatory cervical group and cervical squamous cell carcinoma group were significant difference( P < 0. 05),CINⅠgroup and cervical squamous cell carcinoma group were not significant difference(P > 0. 05),CIN Ⅱ-Ⅲ group and cervical squamous cell carcinoma group were not significant difference( P > 0. 05). Conclusion:DHRS4 gene was highly expressed in human cervical squamous cell carcinoma,and the positive rate of expression with the progression of cervical lesions was gradually increased,which was may related to the occurrence of cervical disease and its malignant transformation.