P16/Ki67在宫颈低级别鳞状上皮内病变中的表达水平及对随访的指导价值

Study on the expression of P16/Ki67 in low-grade squamous intraepithelial lesion and its guidance value for follow-up

目的探讨P16/Ki67在宫颈低级别鳞状上皮内病变(LSIL)中的表达水平,分析其及对随访的指导价值。方法采用P16/Ki67免疫组化双染法检测100例LSIL患者P16/Ki67蛋白的表达水平,根据免疫组化结果分组为双染阳性组、双染阴性组。随访观察2年,比较两组患者的转归结局。结果 LSIL患者中,P16、Ki67及P16/Ki67双染的阳性率分别为48.0%、51.0%及42.0%。CIN I患者P16、Ki67及P16/Ki67双染的阳性率均显著高于湿疣患者(P<0.05)。LSIL患者P16、Ki67表达呈显著正相关性(P<0.05)。双染阴性组的转归结局显著优于双染阳性组(P<0.05)。结论P16/Ki67双染法可作为LSIL病变转归结局的预警指标,可用于随访的分流管理,对于LSIL患者干预或随访策略的制定具有指导意义。

Objective To explore the expression level of P16/Ki67 in low - grade squamous intraepithelial lesions （LSIL） , and to analyze the guidance value for follow - up. Methods P16/Ki -6 7 dual immunohistochemical staining had been performed to detect the expression of P16/Ki67 in 100 patients with LSIL, all these patients were divided into dual stain positive group and dual stain negative group according to the immunohistochemical results. All these patients were followed up for 2 years, and the difference of outcome between these two groups was compared. Results In patients with LSIL, the positive rates of P16 , Ki67 and P16/Ki67 dual staining were 48. 0% , 51.0% and 42. 0% respectively. The positive rates of P16, Ki67 and P16/Ki67 dual staining in patients with CIN I were significantly higher than those of patients with condyloma （P 〈 0. 05）. The positive expression of P16 and Ki67 was significantly correlated in LSIL patients （ P 〈0. 0 5） . The outcome in dual staining negative group was superior to that of dual staining positive group （ P 〈0. 0 5） . Conclusion P16/Ki67 dual staining can be used as earlywarning indicator of outcome and distribution management of follow - up in patients with LSIL, and it has guiding significance for intervention ofollow - up strategy.