阿胶酶解液相对分子质量分布及其补血升白作用

Distribution of Relative Molecular Mass of Asini Corii Colla Hydrolyzate and Investigation of Its Blood Enrichment and Leukogenic Effect

目的:研究阿胶酶解液的相对分子质量分布及其补血、升白的作用。方法:采用TSK-GEL G2000SWXL色谱柱(7.8 mm×300 mm,5μm),流动相0.1 mol·L^(-1)NaCl+0.05%NaN_3+0.1 mol·L^(-1)磷酸盐缓冲液(pH 6.7),流速0.5 m L·min^(-1),检测波长280 nm,对阿胶原液、阿胶仿生酶解液、阿胶胰蛋白酶酶解液进行相对分子质量分布的测定;采用放血法致小鼠血虚模型及环磷酰胺致小鼠白细胞减少模型,观察阿胶酶解液的补血升白作用。结果:阿胶所含蛋白及多肽的相对分子质量>6.64×10~4Da,胰蛋白酶酶解液及仿生酶解液的相对分子质量主要集中在3.7×10~3Da左右;阿胶仿生酶解液及胰蛋白酶酶解液可使血虚模型小鼠血红蛋白(Hb),红细胞计数(RBC)水平显著提高,使白细胞减少模型小鼠的白细胞计数(WBC),RBC和Hb水平显著提高。结论:阿胶经酶解后相对分子质量减小且具有显著的补血升白作用,胰蛋白酶酶解与仿生酶解效力大致相当。

Objective ： To study on distribution of relative molecular mass of Asini Corii Colla hydrolyzate and discuss its blood enrichment and leukogenic effect. Method： TSK-GEL G200OSWXL column （7.8 mm × 300 mm） was employed, mobile phase was consisted of 0. 1 mol· L^-1 NaCl ＋ 0.05% NaN3 ＋ 0. 1 mol ·L^-1 phosphate buffer solution （ pH 6.7） and detection wavelength was set at 280 nm ; distributions of relative molecular mass of Asini Corii Colla concentrate, bionic hydrolysate and trypsin hydrolysate were determined. The bloodletting method was adopted to establish blood deficiency mice model, leukopenia model induced by cyclophosphamide in miee were used, blood enrichment and leukogenie effect of Asini Corii Colla hydrolyzate was observed. Result： Relative molecular mass of protein and polypeptide in Asini Corii Colla was greater than 6.64 × 10^4 Da, relative molecular mass of bionic hydrolysate and trypsin hydrolysate of Asini Corii Colla mainly concentrated in about 3.7 × 10^3 Da. Bionic hydrolysate and trypsin hydrolysate of Asini Corii Colla could significantly improve hemoglobin （Hb） andred cell count （RBC） of blood deficiency model mice, and they could significantly increase white blood cell count （WBC） , RBC and Hb levels of leukopenia model mice. Conclusion： After the enzymolysis, relative molecular mass of Asini Corii Colla decreases and its blood enrichment and leukogenic effect is significant, effect of bionic hydrolysate and trypsin hydrolysate is roughly equivalent.