黑龙江凤凰山不同海拔及不同采收期辽东楤木叶中2种皂苷类成分含量的比较

Content of Two Saponins in Leaves of Aralia elata from Different Altitudes and Different Gathering Periods of Fenghuang Mountain

目的:比较黑龙江凤凰山地带不同海拔及不同采收期辽东楤木叶中三萜皂苷3-O-[β-D-吡喃葡萄糖基(1→2)][β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基-刺囊酸-28-O-β-D-吡喃葡萄糖酯(简写为ECA)和3-O-β-D-吡喃葡萄糖基-(1→3)-β-D-吡喃葡萄糖基-(1→3)-β-D-吡喃葡萄糖基-齐墩果酸-28-O-β-D-吡喃葡萄糖酯(以下简称为OLA)的含量,了解这一区域采收辽东楤木叶的最佳时间和海拔高度。方法:用反相高效液相色谱-蒸发光散射检测(HPLC-ELSD)法测试,色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),柱温30℃;流动相为乙腈、水二元梯度系统;进样量10μL,流速1.0 m L·min^(-1);载气流速3.2 L·min^(-1);漂移管温度110℃。结果:两种三萜皂苷在各自测定的范围内均呈良好线性关系(r均为0.999 8),平均加样回收率分别为97.71%,98.06%。各批次供试品中OLA的含量都远高于ECA。从6月初至9月初,两种皂苷含量几乎都呈递增趋势,6月至8月增幅很大,差别显著,8月至9月增幅较小,甚至略减;不同海拔高度条件下同时期的叶中两种皂苷含量没有显著性差异。结论:所用测试方法简单、快捷、重复性好,可为凤凰山地带辽东楤木叶的质量控制提供依据。

Objective： To compare the contents of 3-0-[β-D-glucopyranosyl-（ 1 →2 ） ] [β-D- glucopyranosyl-（ 1→3 ） ] -β-D-glucopyranosyl echinocystic acid-28-O-β-D-glucopyranosyl ester （ ECA for short） and 3-O-β-D-glucopyranosyl- （ 1 →3 ） -β-D-glucopyranosyl- （ 1 →3 ） -β-D-glueopyranosyl oleanolic acid-28-O-β-D- glucopyranosyl ester （ OLA for short） in leaves of Aralia elata collected from Fenghuang mountain of Heilongjiang Province in different altitudes and different gathering periods. Method： HPLC-ELSD was used to determine the content. The column was Diamonsil C18 （4.6 mm× 250 mm, 5 μm）. The column temperature was 30 ℃. The mobile phase was the mixture liquid of acetonitrile and water for gradient elution. The sample size was 10 μL, and the flow rate was 1.0 mL·min^-1 The detector was HPLC-ELSD, the temperature of drift tube was 110 ℃, and the flow rate of carrier gas was 3.2 L· min ^- 1 Result： The two triterpenoid saponins showed a good linearity （ r = 0. 999 8 ） within their detection range, and the average recovery rate were 97.71% and 98.06%, respectively. The results showed that the content of September, the contents of the two OLA was higher than ECA in various batches of samples. From June to saponins showed an increasing trend. The contents increased significantly from June to August, but slowly from August to September. In addition, there were no significant difference in content of the two saponins between high altitude and low altitude. Conclusion： The method is simple, rapid and reproducible, and can provide a basis for the quality control of leaves of A. elata from Fenghuang Mountain.