摘要
目的:对中药乳香的质量标准进行提高,使中药乳香饮片及其制剂高效和安全应用。方法:从乳香质量标准和刺激性两方面进行研究,采用薄层色谱法对乳香中乳香酸类成分进行鉴别,采用苯酚-硫酸法测定乳香总多糖含量,采用气相色谱法对乳香挥发油主要药效成分乙酸辛酯含量进行测定,并采用动物实验对乳香的刺激性进行研究。结果:乳香薄层色谱中,乳香酸类成分在与对照品色谱相应的位置上显相同颜色的暗斑,空白对照无干扰;乳香中总多糖平均加样回收率为99.6%,RSD 1.2%,平均质量分数为7.0%;乳香挥发油中乙酸辛酯平均加样回收率为100.16%,RSD 0.9%,平均质量分数为0.08%;乳香生饮片挥发油有明显刺激性。结论:研究建立的乳香中乳香酸类成分薄层色谱鉴别方法、乳香总多糖和乙酸辛酯含量测定方法简便、准确,达到提高乳香中药饮片质量标准的目的,并为乳香提取物及其制剂的质量标准和高效安全用药提供依据和保障。
Objective: To improve the quality standards of Olibanum, and achieve efficient and safe application of Olibanum decoction pieces and its preparations. Method: Olibanum was studied from quality standard and irritation aspects. Boswellic acids in Olibanum were analyzed by TLC, and the total polysaccharide content was determined by phenol sulfuric acid method; gas chromatography was used to determine the content of octyl acetate in the volatile oil of Olibanum, and the irritation of Olibanum was studied by using animal experiment. Result: In the thin layer chromatography of Olibanum, the components of boswellic acid showed the same color spots in the corresponding positions of the chromatogram for reference substances, with no interference in the blank control. The average recovery rate of total polysaccharides in Olibanum was 99.6% , with RSD of 1.2% and average mass fraction of 7.0%. The average recovery rate of octyl acetate in Olibanum volatile oil was 100. 16% , with RSD of 0.9% , and average content mass fraction of 0.08%. In addition, volatile oil of Olibanum pieces had irritation. Conclusion: The TLC method for identifying boswellic acids and the determination method for total polysaccharides and octyl acetate in Olibanum were simple and accurate. These methods can be used to improve the quality standards of Olibanum decoction pieces, and provide the basis and protection for quality standards of Olibanum extracts and formulations as well as their efficient and safety use.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第12期77-81,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81273883)
关键词
乳香
质量标准
薄层鉴别
含量测定
刺激性
Olibanum
quality standard
TLC
determination of content
irritation