牛磺鹅去氧胆酸对佐剂性关节炎大鼠成纤维样滑膜细胞ICAM-1表达的影响

Effect of taurochenodeoxycholic acid on the expression of ICAM-1 in fibroblast-like synoviocytes of adjuvant arthritis in rats

为了研究牛磺鹅去氧胆酸(taurochenodeoxycholic acid,TCDCA)对佐剂性关节炎模型大鼠成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)中细胞间黏附分子-1(intercellular cell adhesion molecule-1,ICAM-1)表达的影响,采用qPCR与ELISA法检测了FLS中ICAM-1的表达量,结果表明,1×10^(-6)~1×10^(-4) mol/L TCDCA在基因与蛋白水平均能够显著或极显著抑制IL-1β刺激下FLS中ICAM-1的表达(P<0.05或P<0.01),且该抑制作用可被糖皮质激素受体(glucocorticoid receptor,GR)阻断剂米非司酮(RU486)拮抗,提示TCDCA对佐剂性关节炎的治疗作用与抑制GR活性进而抑制ICAM-1表达有关。

To investigate the effect of taurochenodeoxyeholic acid on the expression of intercellular cell adhesion molecule-l（ICAM-1） in fibroblast-like synoviocytes（FLS） of adjuvant arthritis（AA） in rats, qPCR and ELISA were used to measure the mRNA and protein expression of ICAM-1 ,re- spectively. The results showed that the gene and protein expression of ICAM-1 were inhibited sig- nificantly by TCDCA at the concentration of 1 × 10^-6 -1 × 10^-4 mol/L,and the inhibition of TCD- CA could be blocked by glucocorticoid receptor（GR） inhibitor mifepristone（RU486）, which sug- gests that the mechanism of TCDCA treatment for AA was related to inhibition of ICAM-1 ex- pression mediated by GR.