围手术期食管鳞状细胞癌患者血清UHRF1水平变化

Changes of serum UHRF1 levels in perioperative patients with esophageal squamous cell carcinoma

目的检测食管鳞状细胞癌(ESCC)患者血清泛素样含PHD和环指域1(UHRF1)水平,分析其在不同临床病理参数亚组间的差异以及围手术期的变化。方法收集130例ESCC患者术前血清,另收集其中62例患者的术后1周血清以及14例患者术后1周和2周血清,以67例体检健康者作为对照组。同时收集患者临床病理资料。用ELISA法检测血清UHRF1含量,采用独立样本t检验、配对t检验及单因素方差分析比较不同组间水平差异。结果 130例ESCC患者术前血清UHRF1含量明显高于健康人对照组(t=7.680,P<0.01);在不同年龄、性别、肿瘤部位和大体分型间UHRF1水平差异无统计学意义(P>0.05),不同肿瘤大小、分化程度、浸润深度、淋巴结转移情况、pTNM分期间血清UHRF1水平差异有统计学意义(P<0.05)。62例患者术后UHRF1水平较术前明显降低(t=5.530,P<0.01),且与健康人对照组差异无统计学意义(t=1.622,P>0.05);14例患者术前、术后1周、术后2周血清UHRF1水平逐渐降低,差异有统计学意义(F=7.595,P<0.01)。结论血清UHRF1可作为围手术期动态监测的潜在指标。

Objective To detect serum ubiquitin-like with PHD and ring finger domains 1 （ UHRF1 ） levels in the patients with esoph- ageal squamous cell carcinoma （ ESCC）, and analyze their differences among different clinicopathological features subgroups and chan- ges during the perioperative period. Methods Serum samples from 130 preoperative ESCC patients, 62 patients 1 week after opera- tion, 14 patients 1 week and 2 weeks after operation and 67 healthy controls, and clinicopathological data from ESCC patients were col- lected. Serum UHRF1 levels were detected by^ELISA, and the differences among different groups were analyzed with independent t test, paired t test or one-way ANOVA. Results Serum UHRF1 levels in 130 preoperative ESCC patients were significantly higher than that in healthy controls （t = 7. 680, P 〈 0.01 ）, and they were related to the ESCC patients＇ tumor size, differentiation degree, tumor invasion, lymph nodes metastasis and pTNM stage （P 〈 0.05 ）, but unrelated to the patients＇ age, gender, tumor location and types （P 〉 0.05 ）. Serum UHRF1 levels in 62 postoperative patients were significantly lower than that before operation （ t = 5. 530, P 〈 0.01 ）, but similar to that in healthy controls （t = 1. 622, P 〉0.05）. The serum UHRF1 levels before operation, 1 week after opera- tion and 2 weeks after operation in 14 ESCC patients decreased gradually （ F = 7. 595, P 〈 0.01 ）. Conclusion Serum UHRF1 levels may be a potential biomarker for dynamically monitoring perioperative ESCC patients.