IL-17基因多态性位点与新疆地区慢性阻塞性肺病的风险关系

Correlation of IL-17 gene polymorphism with chronic obstructive pulmonary disease in Xinjiang aera

目的探讨IL-17基因核苷酸多态性与新疆地区慢性阻塞性肺病(COPD)的风险关系。方法采用多重单碱基延伸单核苷酸多态性(single nucleotide polymorphism,SNP)分型技术(SNa Pshot)对149例COPD患者及97例健康人IL-17A基因rs2275913、rs3819024、rs3819025、rs4711998、rs8193036 5个SNP位点及IL-17F基因rs12203582、rs1266828、rs7771466、rs9382084 4个SNP位点进行分型,并比较不同基因型和等位基因与COPD的风险关系。结果健康人与稳定期COPD患者在IL-17A的5个SNP位点及IL-17F的4个SNP位点均未见差异。其位点的变异、突变基因型分布与COPD的易感性亦无明显差异,对IL-17F及IL-17A基因的SNP进行连锁不平衡反应后的分析结果表明,IL-17A基因CAA单体型在COPD与健康人间差异存在统计学意义(OR=0.244,95%CI:0.057~1.043,P<0.05)。结论 IL-17F及IL-17A基因多态性与COPD患病无直接风险关系,但在IL-17A基因单体型CAA改变可能与新疆地区COPD易感性有关。

Objective To investigate the correlation of IL-17 gene polymorphism with chronic obstructive pulmonary diseases （COPD） in Xinjiang area. Methods Five single nucleotide polymorphism （SNP） loci of IL-17A gene, including rs2275913, rs3819024, rs3819025, rs4711998 and rs8193036, and 4 SNP loci of IL-17F gene such as rs12203582, rs1266828, rs7771466 and rs9382084 from 149 COPD patients and 97 healthy controls were performed typing using the multiple single-base extension SNP （SNaPshot） tech- nology, and the risk correlations of different genotypes and alleles of these loci with COPD were analyzed. Results There was no difference in the SNP of 5 IL-17A gene loci and 4 IL-17A gene loci between stable COPD patients and healthy controls, and the muta- tion and genotype distribution of these loci were not correlated with COPD susceptibility. However, the linkage disequilibrium analysis showed that there was significant difference in the distribution of IL-17A gene haplotype CAA between COPD patients and healthy con- trols （OR=0.244, 95%CI： 0.057-1.043, P〈0.05）. Conclusion The IL-17F and IL-17A gene polymorphisms are not correlated with the risk of COPD, but the haplotype CAA of IL-17A gene may be related to the susceptibility of COPD in Xinjiang area.