喷砂酸蚀混合碱热处理后纯钛表面生物活性的研究

Bioactivity of the pure titanium surface treated with sand blasting-acid-alkali heat treatment

目的研究喷砂酸蚀混合碱热处理后的纯钛表面其生物活性。方法将经抛光(M)、喷砂酸蚀(SLA)、喷砂酸蚀混合碱热(AHH)处理后的纯钛试件分为3组。扫描电子显微镜(SEM)及能谱分析(EDS)对3组表面结构和表面化学元素及其含量进行分析;荧光显微镜下观察成纤维细胞在样品表面的粘附及铺展情况;并将各组试件浸泡模拟体液(SBF)中观察羟基磷灰石沉积情况。结果纯钛表面微纳复合结构中,微米孔直径3~5μm,纳米孔直径100~200 nm,同时在试件表面引入钙钠元素。成纤维细胞粘附:DAPI染色后细胞核呈蓝色荧光,罗丹明B染细胞骨架红色,SLA组、AHH组细胞铺展较M组好,呈空间铺展;且AHH组表面细胞数量明显多于其他两组。第1周时,AHH组表面沉积磷灰石明显可见,而未在M、SLA组检测到羟基磷灰石。结论喷砂酸蚀混合碱处理后的纯钛表面,表面活性好,有助于促进成纤维细胞早期粘附及其在空间上的铺展,同时又促进羟基磷灰石的沉积,进而体现了良好的生物性能,可以为种植体表面处理方法提供参考。

Objective To study the bioactivity of the pure titanium surface treated with sand blasting and acid-alkali heat treatment. Methods The specimens were divided into 3 groups which were respectively treated by machined （M）, sand blasting-acid etching （SLA） and sand blasting-acid-alkali heat treatment（AHH）. Scanning Electron Microscope（SEM） and Energy Dispersive Spectrometer （EDS） were separately used to record and analyze surface topography and surface elements as well as its content. Besides, the adhesion and spreading of fibroblastic cytoskeleton on the specimen surface were detected by Fluorescence microscope. In addition, the deposition of hydroxyapatite was inspected by SEM in simulated body fluid （SBF）. Results The pure titanium decorated with micro/nano pores （diameter 3 - 5 μm; 100 -200 nm） brought in Calcium ion and Sodium ion. The nucleus was dyed blue by DAPI and cytoskeleton turned into red by Rhodamine B. The cell spreading of SLA and AHH was better than M, which presented spacial spreading. A dense hydroxyapatite layer clearly formed on AHH surface in the first week. However, little was detected on M and SLA. Conclusion The pure titanium surface treated with sand blasting-acid-alkali heat treatment displays better biological activity. Not only does it promote fibroblasts adhesion, but also accelerates forming hydroxyapatite. It may provide a better surface treatment for implant design.