黄瓜Ca^(2+)家族序列特征及其表达特性分析

Analysis on sequence characteristics and expression of Ca^(2+) family of cucumber

通过黄瓜基因组数据库,采用生物信息学方法获得15个黄瓜Ca^(2+)家族序列,并对其染色体位置、基因组位置、氨基酸长度、转录起始位点和Pfam匹配结构域数进行预测;利用RT-PCR方法对所有搜索到的Ca^(2+)家族基因进行组织特异性分析;对黄瓜植株进行低温处理,分析黄瓜Ca^(2+)基因家族的表达情况。结果显示:15个基因中除Csa021245未定位以外,其余均匀分布在1~6号染色体上,一致性为47.67%;Csa001688、Csa009857和Csa009360等3个基因的5′区出现CpG岛结构;组织表达特异性分析结果显示,Csa001688、Csa006826、Csa010172和Csa016434在叶中表达量最高,Csa003758、Csa010172和Csa018949在根中表达量最高;进化树分析结果显示,只有Csa010172和Csa009360与拟南芥AtACA2亲缘关系最近,这些基因可能在Ca^(2+)家族信号传导过程中起着主要作用;实时定量表达分析表明,Ca^(2+)基因家族参与黄瓜对低温胁迫的应答。

Fifteen sequences of Ca^2＋ family in cucumber were indentified from Cucumber Genome Database using bioinformatics methods, and the chromosomal location, genomic position, amino acid length, transcription initiation site and Pfam matching domain were predicted. At the same time, sequence similarity and phylogenetic tree were analyzed. Cucumber plants were exposed to low temperature, and the expression of Ca^2＋ gene family was analyzed. The results showed that 5 sequences distributed on the first chromosome, 3 sequences distributed on the second chromosome, 2 sequences distributed on the fourth chromosome, 1 sequence was not located, the remaining sequences evenly distributed on other chromosomes. With RT-PCR technique, the expressions of Csa001688, Csa006826, CsaOlO172 and Csa016434 in leaves were higher, the expressions of Csa003758, Csa010172 and Csa018949 in roots were higher. Phylogenetic tree analysis showed that, only Csa010172 and Csa009360 were close with Arabidopsis AtACA2, these genes may play important roles in Ca2＋ family signal transduction. Real - time quantitative expression analysis showed that Ca^2＋ gene family was involved response to low temperature stress in cucumber.