摘要
以苯甲酸、芳氨基硫脲或单缩二氨基硫脲为原料,合成得到5个取代硫脲基苯甲酰基硫脲衍生物,经红外、质谱、核磁共振氢谱和元素分析确认了化合物的结构。测定其清除超氧自由基(O_2^-·)、羟自由基(·OH)和2,2-二苯基-1-苦味酰基自由基(DPPH·)的活性及总还原能力,并与维生素C的活性进行比较。以水稻和黄瓜幼苗为试材,用生长测试法、超氧歧化酶(SOD)活性测定法以及过氧化物酶(POD)活性测定法研究这些化合物作为外源抗氧化剂对植物的生长调节作用。结果表明,在测定浓度下,这些化合物均具有清除自由基活性,并对水稻和黄瓜幼苗的生长发育有促进作用,且能激起SOD和POD的活力。
Five thiourea benzoylthiourea derivatives were synthesized by using benzoic acid, arylthiosemicarbazide or thiosemicarbazones as raw materials. Their structures were characterized by UV, IR, 1H-NMR and MS. Their scavenging effects on the superoxide radical ( O2^-· ) , hydroxyl radical (·OH ) and DPPH·radical and their total reduction capacity were compared with ascorbic acid ( Vc ) as the reference material. As exogenous antioxidants, their promoting effects on plant growth of rice and cucumber were tested by the methods of growth test, superoxide dismutase ( SOD ) and peroxidase ( POD ) activity determination. The results showed that at the determinated concentration, these compounds all had scavenging effects on these radicals, and also could promote the growth of rice and cucumber, at the same time increase their SOD and POD activity.
出处
《广东农业科学》
CAS
2017年第3期113-119,共7页
Guangdong Agricultural Sciences
基金
广东省科技计划项目(2014A030304070)
关键词
苯甲酰基硫脲
合成
抗氧化
植物生长调节
benzoylthiourea
synthesis
antioxidant activity
plant growth regulator