饲料蛋白中添加虾蛋白肽对珍珠龙胆石斑鱼幼鱼生长性能和抗氧化指标的影响

Effects of dietary shrimp protein-peptides on growth performance and antioxidant indices of juvenile Epinephelus lanceolatus×E. fuscoguttatuss under two dietary protein levels

为研究两种蛋白水平下添加虾蛋白肽对珍珠龙胆石斑鱼幼鱼生长性能和抗氧化指标的影响,设计6种饲料,以440、480 g/kg两种饲料蛋白与0、10和20 g/kg 3种虾蛋白肽添加量配制。结果显示,相同蛋白水平下,石斑鱼的终末均重和增重率均随着虾蛋白肽水平的上升而先上升后下降,10 g/kg虾蛋白肽组显著高于0、20 g/kg虾蛋白肽组;虾蛋白肽添加量相同时,480 g/kg饲料蛋白组显著高于440 g/kg饲料蛋白组。蛋白质效率、肌肉粗蛋白和粗脂肪含量、血清AST和ALT活力、肝脏CAT和T-AOC活力的变化规律与增重率类似。饵料系数、血糖含量和肝脏MDA含量的变化规律则与之相反。试验结果表明,在不同蛋白水平的饲料中添加虾蛋白肽均能促进珍珠龙胆石斑鱼幼鱼的生长,并提高其抗氧化能力,但添加量过高会产生抑制作用,因此,虾蛋白肽适宜添加量为10 g/kg。

To investigate the effects of dietary shrimp protein-peptides （ SPP ） on growth performance and antioxidant indices of juvenile Epinephehts lanceolatus × E. fuscoguttatuss under two dietary protein levels, six diets were formulated to contain two crude protein （ CP ） concentrations （ 440 g/kg and 440 g/kg ） and three SPP levels （ 0 g/kg, 10 g/kg and 20 g/kg ） . Each diet was randomly assigned to triplicate treatments of 20 fishes for 8 weeks,and the initial body weight of fish was 13.67 （ ±0.11 ） g. The results indicated that when the dietary protein concentration was the same, the final body weight （ FBW ） and weight gain rate （ WGR ） significantly increased with SPP level increasing to 10 g/kg, beyond which a decrease was observed. When the SPP level was the same, FBW and WG of 480 g/kg CP group were significantly higher than those of 440 g/kg CP group. The change trends of protein efficiency ratio, muscle crude protein and crude fat contents, serum AST and ALT activities, hepatic CAT and T-AOC activities were similar with WGR. Meanwhile, the change trends of feed conversion ratio, serum glucose content and liver MDA content were the opposite with WGR. In summary, adding SPP in diets containing different protein concentrations could promote the growth performance of juvenile E. lanceolatus × E. fuscoguttatuss, and increase its antioxidant capacity, but adding too high level of SPP would cause inhibition. Therefore, the suitable SPP level was is 10g/kg.