溴氰菊酯对中华绒螯蟹主要免疫指标的影响

Effects of deltamethrin on main immune parameters of Chinese mitten crab,Eriochier sinensis

为揭示溴氰菊酯对中华绒螯蟹免疫功能的影响,测定了在不同浓度溴氰菊酯(0、0.19、0.30、0.41、0.52μg/L)条件下中华绒螯蟹总血细胞数量(THC)、吞噬活性、酚氧化酶(PO)活性、酸性磷酸酶(ACP)活性和碱性磷酸酶(AKP)活性5项主要免疫指标的变化情况。结果表明,溴氰菊酯对中华绒螯蟹免疫有显著影响,低浓度处理(0.19、0.30μg/L)引起THC药后6 h和12 h出现极显著上升,随后逐渐恢复;而高浓度处理(0.41、0.52μg/L)则在药后24 h后持续下降,药后96 h极显著低于对照。各浓度处理均对血细胞吞噬活性有抑制效应,且随着浓度升高抑制效果越强。0.52μg/L处理PO活性药后6 h出现极显著上升,随后逐渐下降,药后96 h处理间无显著差异;而ACP活性、AKP活性药后6 h出现明显下降,各时间段均极显著低于对照。

In this research, the effects of deltamethrin on immune parameters were revealed by dectecting the changes of total hemocytes count （ THC ） , phagocytic activity, phenoloxidase （ PO ） activity, acid phosphatase （ACP） activity and alkaline phosphatase （ AKP ） activity in hemolymph of Chinese mitten crab under different concentrations of deltamethrin （0, 0.19, 0.30, 0.41,0.52 μg/L ）exposure. The results showed that dehamethfin had evident effects on immune parameters of Chinese mitten crab, THC increased after 6 h and 12 h exposure to low concentrations of deltamethrin including 0.19 μg/L and 0.30 μg/L, and then decreased to normal level at 96 h. On the contrary, high concentration （ 0.41 μg/L and 0.52 μg/L ） exposure declined the THC after 24 h, which was significantly lower than that of control group at 96 h. The phagocytic activity decreased under each concentration and the higher concentration made a higher inhibition of phagocytic activity. After exposure to 0.52μg/L deltamethrin, PO activity increased significantly at 6 h and then decreased gradually till 96 h, which had no significant difference among each group. However, both ACP and AKP activity decreased at 6 h exposure to 0.52μg/L deltamethrin and was significantly lower than those of control at each time point.