摘要
目的:研究马齿苋多糖(POP)对免疫抑制小鼠细胞因子的影响,并探讨其对小鼠免疫功能作用机制。方法:注射环磷酰胺(CTX)建立环磷酰胺诱导免疫抑制小鼠模型,灌胃(ig)给予不同剂量的POP(180和90 mg·kg^(-1))和阳性对照药香菇多糖片(100 mg·kg^(-1)),通过计算小鼠模型前后脾脏指数和胸腺指数。MTT法测定淋巴细胞增殖反应,双抗夹心ELISA法测定小鼠血清中细胞因子IL-2,IL-4,IL-10,IFN-γ,NK和LAK的含量。结果:POP可增强CTX所致小鼠自然杀伤细胞和淋巴因子激活的杀伤细胞活性,并能增加血清细胞因子IL-2,IL-4,IL-10和IFN-γ的含量;POP可提高免疫抑制小鼠的脾脏指数和胸腺指数。结论:马齿苋多糖能明显提高小鼠机体的免疫能力,恢复由CTX诱导的小鼠免疫抑制状态,这可能是其抗肿瘤活性的作用机制之一。
Objective: To investigate the effect of portulaca oleracea polysaccharide (POP) on immunologic function in immunosuppressed mice and the possible mechanism. Methods: The immunosuppression was induced by cyclophosphamide (CTX) in mice. POP (180 and 90 mg·kg^-1) and the positive control agent lentinan (100 mg·kg^-1) were orally administered, respectively. Thymus and spleen indexes were determined. Proliferation of T and B lymphoeytes was measured by MTT assay. IL-2, ELISA. Results: POP enhanced the activity of NK and IL-4, IL-10, and IFN-γ in the serum were measured by LAK cells, and significantly increased the levels of IL-2, IL-4, IL-10, and IFN-γ in serum. It also increased the index thymus immunosuppression and myelosuppression caused by CTX in mice, thus may and spleen. alleviate the Conclusion : side effects of POP improves chemotherapy.This effect may be an improtant antitumor mechanism of POP.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2017年第11期1296-1300,共5页
Chinese Journal of New Drugs
基金
国家自然科学基金委员会-河南省人民政府人才培养联合基金资助项目(U1304824)
中国博士后科学基金特别资助项目(2015T80772)
关键词
马齿苋多糖
免疫抑制
细胞因子
portulaca oleracea polysaccharide
immunosuppression
immunosuppression cytokines