马齿苋多糖对环磷酰胺模型小鼠免疫功能的影响

Effect of portulaca oleracea polysaccharide on immunological function in mice with cyclophosphamide-induced immunosuppression

目的:研究马齿苋多糖(POP)对免疫抑制小鼠细胞因子的影响,并探讨其对小鼠免疫功能作用机制。方法:注射环磷酰胺(CTX)建立环磷酰胺诱导免疫抑制小鼠模型,灌胃(ig)给予不同剂量的POP(180和90 mg·kg^(-1))和阳性对照药香菇多糖片(100 mg·kg^(-1)),通过计算小鼠模型前后脾脏指数和胸腺指数。MTT法测定淋巴细胞增殖反应,双抗夹心ELISA法测定小鼠血清中细胞因子IL-2,IL-4,IL-10,IFN-γ,NK和LAK的含量。结果:POP可增强CTX所致小鼠自然杀伤细胞和淋巴因子激活的杀伤细胞活性,并能增加血清细胞因子IL-2,IL-4,IL-10和IFN-γ的含量;POP可提高免疫抑制小鼠的脾脏指数和胸腺指数。结论:马齿苋多糖能明显提高小鼠机体的免疫能力,恢复由CTX诱导的小鼠免疫抑制状态,这可能是其抗肿瘤活性的作用机制之一。

Objective： To investigate the effect of portulaca oleracea polysaccharide （POP） on immunologic function in immunosuppressed mice and the possible mechanism. Methods： The immunosuppression was induced by cyclophosphamide （CTX） in mice. POP （180 and 90 mg·kg^-1） and the positive control agent lentinan （100 mg·kg^-1） were orally administered, respectively. Thymus and spleen indexes were determined. Proliferation of T and B lymphoeytes was measured by MTT assay. IL-2, ELISA. Results： POP enhanced the activity of NK and IL-4, IL-10, and IFN-γ in the serum were measured by LAK cells, and significantly increased the levels of IL-2, IL-4, IL-10, and IFN-γ in serum. It also increased the index thymus immunosuppression and myelosuppression caused by CTX in mice, thus may and spleen. alleviate the Conclusion ： side effects of POP improves chemotherapy.This effect may be an improtant antitumor mechanism of POP.