DCE-MRI定量参数K^(trans)值评估抗血管生成药物治疗裸鼠胃癌原位移植瘤的疗效

K^(trans) of dynamic contrast enhanced MRI in evaluation of anti-angiogenic effects on nude mice with orthotopic transplantation tumor model of gastric cancer

目的分析MR动态增强扫描(DCE-MRI)定量参数Ktrans值评估甲磺酸阿帕替尼治疗裸鼠胃癌原位移植瘤的疗效。方法建立裸鼠胃癌原位移植瘤模型后,随机分为治疗组(n=15,胃内灌注甲磺酸阿帕替尼100mg/kg体质量)和对照组(n=15,胃内灌注无菌去离子水),治疗18天后行DCE-MRI,并定量测量Ktrans值。检查结束后,取出瘤体测量微血管密度(MVD)值和血管内皮生长因子(VEGF)水平,并对比组间差异。结果与对照组相比,治疗组MVD值(χ2=4.89,P<0.05)、VEGF水平明显减低(χ2=8.69,P<0.01),治疗组Ktrans值[(0.63±0.05)/min]明显低于对照组[(1.66±0.23)/min;t=17.05,P<0.01]。结论 DCE-MRI定量参数Ktrans值可作为胃癌抗血管生成药物甲磺酸阿帕替尼疗效的评估指标。

Objective To assess the feasibility of K value of dynamic contrast enhanced MRI （DCE-MRI） in evaluation of anti-angiogenic effects on nude mice with orthotopic transplantation tumor model of gastric cancer. Methods Nude mice with orthotopic transplantation tumor model of gastric cancer were randomly assigned to two groups： Treatment group （n = 15）, mice were given apatinib intragastrically for 18 days （100 mg/kg）, and control group （n= 15）, mice were given ddH202 in the same manner. After 18 days, DCE-MRI was performed and K value was measured. Then the tumors were dissected from the adjacent tissues in order to detect the microvessel density （MVD） and vascular endothelial growth factor （VEGF） expression levels. MVD and VEGF expression level were compared between treatment group and the con- trol group. Results MVD （x22 =4.89, P（0.05） and VEGF expression level （x2=8.69, P（0.01） of treatment group were much lower than those of control groups. The Kt value of treatment group was significantly lower than that of con- trol groups （[0.63±0.05]/min vs [1.66±0.23]/min, t=17.05, P〈0.01）. Conclusion The value of k in DCE-MRI can be utilized to assess the effects of apatinib on nude mice with orthotopic transplantation model of gastric cancer.