广西毛冬青的化学成分研究

Chemical constituents from Ilex pubescens

目的研究广西毛冬青Ilex pubescens var.kwangsiensis根的化学成分。方法利用反复硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱法、高压快速制备色谱及半制备高效液相色谱等方法分离纯化,通过理化常数和NMR、MS波谱等方法鉴定化合物结构。结果从广西毛冬青甲醇提取物中分离得到14个化合物,分别鉴定为丁香苷(1)、3,4-二羟基苯乙醇(2)、橄榄苦苷(3)、红景天苷(4)、木樨榄苷-11-甲酯(5)、(8E)-女贞子苷(6)、(8Z)-ligstroside(7)、oleoacteoside(8)、oleoside dimethyl ester(9)、olivil-4′-O-β-D-glucoside(10)、(+)-cyclo-olivil-6-O-β-D-glucoside(11)、(+)-cyclo-olivil-4′-O-β-Dglucoside(12)、ligstroside(13)、wilfordiol B(14)。结论化合物2、4、12~14为首次从冬青属植物中分离得到,化合物1、3、5~11为首次从本植物中分离得到。

Objective To investigate the chemical constituents from the roots of Ilex pubescens. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, high pressure flash chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results Fourteen compounds were identified as syringin（1）, 3,4-dihydroxyphenyl ethanol（2）, oleuropein（3）, salidroside（4）, oleoside 11-methyl ester（5）,（8E）-nuezhenide（6）,（8Z）-ligstroside（7）, oleoacteoside（8）, oleoside dimethyl ester（9）, olivil-4′-O-β-D-glucoside（10）,（＋）-cyclo-olivil-6-O-β-D-glucoside（11）,（＋）-cyclo-olivil-4′-O-β-D-glucoside（12）, ligstroside（13）, and wilfordiol B（14）. Conclusion Compounds 2, 4, and 12—14 are obtained from this genus for the first time, and compounds 1, 3, and 5—11 are obtained from this plant for the first time.